Transcriptome Sequencing and Metabolism Analysis Reveals the role of Cyanidin Metabolism in Dark-red Onion (Allium cepa L.) Bulbs
文献类型： 外文期刊
作者： Zhang, Chunsha 1 ; Li, Xiaojie 1 ; Zhan, Zongxiang 2 ; Cao, Linjiao 4 ; Zeng, Aisong 5 ; Chang, Guojun 6 ; Liang, Yi 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China
2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
3.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
4.Henan Agr Univ, Hort Coll, Zhengzhou 450002, Henan, Peoples R China
5.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China
6.Jiuquan Acad Agr Sci, Vegetable Res Inst, Jiuquan 735000, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2018 年 8 卷
页码：
收录情况： SCI
摘要： Onion (Allium cepa L.) is an important bulbous vegetable crop that possesses important properties related to health as well as extraordinary colors. Naturally white onion bulbs were used in this study to reveal the complex metabolic mechanisms that underlie phenotypic traits, especially bulb pigmentation. Six libraries (three dark-red and three white) were constructed and analyzed to elucidate differences in cyanidin (Cy) metabolism between dark-red and white onion bulbs. Libraries were screened using RNA-sequencing (RNA-seq) to reveal the differentially expressed genes (DEGs) involved in anthocyanin biosynthesis at the transcriptional level. Comparison with the Kyoto Encyclopedia of Genes and Genomes (KEGG) database shows that a total of 27 unigenes participate in onion anthocyanin biosynthesis and 16 DEGs perform critical roles in flavonoid biosynthesis. Expression patterns of color-related flavonoid compounds associated with the onion anthocyanin biosynthesis pathway (ABP) show that flavonoid 3',5'-hydroxylase (F3'5'H) and dihydroflavonol 4-reductase (DFR) genes play crucial roles in the biosynthesis of dark-red bulbs, the expression levels of flavonol synthase (FLS) and DFR genes may act to block blue pigmentation, and the loss of Cy from white onion bulbs might explain multibranching in the synthesis of this compound. Positive variation in the F3'5'H/F3'H ratio also affects onion bulb color diversity. The transcriptome presented here provides a basis for future onion molecular breeding based on variations in the diversity of ornamental plant pigmentation.
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