您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Analysis of Rfo-Mediated Network in Regulating Fertility Restoration in Brassica oleracea

文献类型： 外文期刊

作者： Xing, Miaomiao 1 ; Xu, Yuanyuan 1 ; Lu, Yuyu 1 ; Yan, Jiyong 1 ; Zeng, Aisong 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Peoples R China

关键词： Rfo; Ogura CMS; transcriptomics; fertility restoration; tapetum; Brassica oleracea

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 22 期

页码：

收录情况： SCI

摘要： Ogura cytoplasmic male sterility (CMS) lines play a crucial role in the utilization of heterosis. However, valuable traits, such as disease resistance genes from Ogura CMS hybrids, are challenging to incorporate for germplasm innovation, particularly in cabbage and broccoli. To date, the Rfo-mediated network regulating fertility restoration remains largely unexplored. In this study, we conducted a transcriptomic analysis of broccoli flower buds from Ogura CMS SFB45 and its Rfo-transgenic fertility restoration line, pRfo, at different stages of pollen development. Gene Ontology (GO) terms such as "pollen exine formation", "flavonoid metabolic and biosynthetic processes", and "pollen wall assembly", along with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including "flavonoid biosynthesis", "MAPK signaling pathway-plant", and "ABC transporters", were significantly enriched. We identified five differentially expressed genes (DEGs) involved in tapetum-mediated callose metabolism, thirty-four DEGs related to tapetum-mediated pollen wall formation, three DEGs regulating tapetum programmed cell death (PCD), five MPKs encoding DEGs, and twelve DEGs associated with oxidative phosphorylation. Additionally, yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays demonstrated that RFO directly interacts with ORF138 at the protein level. These findings provide valuable insights into the fertility recovery mechanisms regulated by Rfo in broccoli and offer important clues for breeders aiming to enhance Ogura CMS hybrids in Brassica oleracea.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台