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Potential large scale production of meningococcal vaccines by stable overexpression of fHbp in the rice seeds

文献类型： 外文期刊

作者： Ma, Jian 1 ; Wang, Yunpeng 2 ; Xu, Nuo 3 ; Jin, Libo 3 ; Liu, Jia 4 ; Xing, Shaochen 1 ; Li, Xiaokun 1 ;

作者机构： 1.Jilin Agr Univ, Fac Agron, Changchun 130118, Jilin, Peoples R China

2.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun 130033, Jilin, Peoples R China

3.Wenzhou Univ, Coll Life & Environm Sci, Wenzhou 325035, Zhejiang, Peoples R China

4.Changchun Univ Chinese Med, Sch Pharmaceut Sci, Changchun 130117, Jilin, Peoples R China

关键词： fHbp; Neisseria meningitidis; Oryza sativa L.; Vaccine; Transformation

期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.65； 五年影响因子：1.548 ）

ISSN： 1046-5928

年卷期： 2018 年 152 卷

页码：

收录情况： SCI

摘要： Factor H binding protein (fHbp) is the most promising vaccine candidate against serogroup B of Neisseria meningitidis which is a major cause of morbidity and mortality in children. In order to facilitate large scale production of a commercial vaccine, we previously used transgenic Arabidopsis thaliana, but plant-derived fHbp is still far away from a commercial vaccine due to less biomass production. Herein, we presented an alternative route for the production of recombinant fHbp from the seeds of transgenic rice. The OsrfHbp gene encoding recombinant fHbp fused protein was introduced into the genome of rice via Agrobacterium-mediated transformation. The both stable integration and transcription of the foreign OsrfHbp were confirmed by Southern blotting and RT-PCR analysis respectively. Further, the expression of fHbp protein was measured by immunoblotting analysis and quantified by ELISA. The results indicated that fHbp was successfully expressed and the highest yield of fHbp was 0.52 +/- 0.03% of TSP in the transgenic rice seeds. The purified fHbp protein showed good antigenicity and immunogenicity in the animal model. The results of this experiment offer a novel approach for large-scale production of plant-derived commercial vaccine fHbp.

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