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The Construction of an Engineered Bacterial Strain and Its Application in Accumulating Mercury from Wastewater

文献类型： 外文期刊

作者： Chang, Shenghe 1 ; Wu, Zhengjing 3 ; Sun, Wei 1 ; Shu, Haiyan 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Haikou Expt Stn, Haikou 570102, Hainan, Peoples R China

2.Key Lab Hainan Banana Genet & Breeding, Haikou 570102, Hainan, Peoples R China

3.Henan Univ Sci & Technol, Sch Forestry, Luoyang 471023, Henan, Peoples R China

关键词： engineering bacteria; mercury; accumulation

期刊名称：APPLIED SCIENCES-BASEL （ 影响因子：2.679； 五年影响因子：2.736 ）

ISSN： 2076-3417

年卷期： 2018 年 8 卷 9 期

页码：

收录情况： SCI

摘要： To remove organic and inorganic mercury from wastewater, an engineered bacterial strain, BL21-7, was constructed that contained the artificial operon P16S-g10-merT-merP-merB1-merB2-ppk-rpsT. For BL21-7, the minimum inhibitory concentrations of mercuric chloride, methylmercury chloride and phenylmercury chloride in Luria-Bertani (LB) medium were 100 mu mol/L, 60 mu mol/L and 80 mu mol/L, respectively. After being cultured in three media (liquid LB containing 80 mu mol/L mercuric chloride, 40 mu mol/L methylmercury chloride or 60 mu mol/L phenylmercury chloride) for 72 h, the engineered bacteria accumulated up to 70.5 +/- 1.5 mu mol/L, 33.5 +/- 3.2 mu mol/L and 45.3 +/- 3.7 mu mol/L of mercury, respectively. In the presence of 10 mu mol/L Cd2+, 10 mu mol/L Pb2+ or 10 mu mol/L Cu2+, the accumulation of mercurial derivatives by BL21-7 was not affected. BL21-7 could accumulate mercury well in media with pH values ranging from 5 to 8 and it could work well at temperatures from 25 degrees C to 37 degrees C. After BL21-7 was added to wastewater and cultured for 24 h, approximately 43.7% of the Hg in the wastewater was removed.

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