Enzymatic determination of uric acid using water-soluble CuInS/ZnS quantum dots as a fluorescent probe
文献类型： 外文期刊
作者： Zhang, Fangmei 1 ; Ma, Pinyi 1 ; Deng, Xinyu 1 ; Sun, Ying 1 ; Wang, Xinghua 1 ; Song, Daqian 1 ;
作者机构： 1.Jilin Univ, Coll Chem, Qianjin St 2699, Changchun 130012, Jilin, Peoples R China
2.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Heilongjiang, Peoples R China
关键词： Microwave-based synthesis; Nanoparticle synthesis; Fluorescent probe; Uric acid determination; Human urine and serum
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）
ISSN： 0026-3672
年卷期： 2018 年 185 卷 11 期
页码：
收录情况： SCI
摘要： Glutathione-capped water-soluble CuInS/ZnS quantum dots (QDs) were prepared by a microwave-assisted method. Their fluorescence, with excitation/emission peaks at 380/570 nm, is found to be quenched by hydrogen peroxide (H2O2) that is produced by the ukase catalyzed oxidation of uric acid (UA) and oxygen. The findings are used in a quenchometric method for the determination of UA. The effects of different ligands on the QDs, of pH value, buffers, enzyme ratio and reaction time were optimized. The detection limit for UA is 50 nM which is lower than other QD-based method, and the detection ranges extends from 0.25-4.0 mu M. The assay is simple and sensitive, and no further modification of the QDs is required.
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