Dopamine-modified Mn-doped ZnS quantum dots fluorescence probe for the sensitive detection of tyrosinase in serum samples and living cells imaging
文献类型： 外文期刊
作者： Zhang, Fangmei 1 ; Xu, Longbin 1 ; Zhao, Qingnan 1 ; Sun, Ying 1 ; Wang, Xinghua 1 ; Ma, Pinyi 1 ; Song, Daqian 1 ;
作者机构： 1.Jilin Univ, Coll Chem, Qianjin St 2699, Changchun 130012, Jilin, Peoples R China
2.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Heilongjiang, Peoples R China
关键词： Mn:ZnS QDs;Tyrosinase;Fluorometric assay;Inhibitor screening;Living cells imaging
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）
ISSN： 0925-4005
年卷期： 2018 年 256 卷
页码：
收录情况： SCI
摘要： Tyrosinase (TYR) plays vital roles in melanin biosynthesis and function as an important biomarker for melanoma cancer; and thus has made rapid and sensitive detections for its activity to be on urgent demands. In this study, a facile aqueous fluorescence probe based on dopamine-functionalized-Mn-doped ZnS quantum dots (Dopa-Mn:ZnS QDs) was developed to detect TYR activity. When dopamine moiety in Dopa-Mn: ZnS QDs was oxidized to dopaquinone derivative by TYR, an intra-particle photo-induced electron transfer (PET) process between Dopa-Mn: ZnS QDs and dopaquinone moiety takes place, and as a result, the fluorescence is quenched. Quantitative evaluation of TYR activity was studied in terms of the relationship between fluorescence quenching efficiency and TYR activity. The probe could detect TYR in a broad linear range of up to 1620 U L-1 with a low detection limit of 1.82 U L-1. It was then applied to detect TYR in chicken serum samples, in which, TYR could be recovered by 88.3-100.7%. Finally, the probe demonstrated low cytotoxicity as evaluated by MTT assay and successfully applied to intracellular imaging of A549 cells. This study has indicated potential applications of Dopa-Mn: ZnS QDs probe in monitoring TYR-associated diseases, clinical diagnostics, and drug discovery. (C) 2017 Elsevier B.V. All rights reserved.
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