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Improving medium chain fatty acid production in Escherichia coli by multiple transporter engineering

文献类型： 外文期刊

作者： Wu, Junjun 1 ; Wang, Zhe 1 ; Zhang, Xia 1 ; Zhou, Peng 1 ; Xia, Xiudong 2 ; Dong, Mingsheng 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang Rd, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Agro Prod Proc, Nanjing 210095, Jiangsu, Peoples R China

关键词： Medium chain fatty acids; Oleo-chemicals; Transporter; Efflux pump

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2019 年 272 卷

页码：

收录情况： SCI

摘要： Medium-chain fatty acids (C-6-C-10, MCFAs) represent valuable molecules for food and pharmaceutical industries. However, MCFAs in Escherichia coli induce stresses and toxicity, reducing final yields. These phenotypes could be alleviated if MCFA export from cells was accelerated, yet information on proteins responsible for exporting MCFAs remains limited. Here, a panel of 17 genes or operons was screened. Overexpressing resistance nodulation cell division family transporter acrE, mdtE and mdtC increased MCFA titer by 46.4%, 65.2% and 33.8%, whereas overexpressing transcriptional activators soxS, marA and multidrug efflux pumps cmr, fadL decreased MCFA titer by 42.5%, 27.8%, 37.6% and 29.5%, respectively. Furthermore, the effect of multiple transporter engineering was investigated. Overexpressing acrE, mdtE and mdtC together with deleting cmr increased MCFA production by more than two-fold. This study demonstrated the efficient efflux pump specifically for MCFAs and this multiple transporter engineering strategy can be employed to synthesize other bio-products toxic to microorganisms.

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