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LmCht5-1 promotes pro-nymphal molting during locust embryonic development

文献类型： 外文期刊

作者： Zhang, Tingting 1 ; Liu, Weiwei 1 ; Li, Daqi 1 ; Gao, Lu 1 ; Ma, Enbo 1 ; Zhu, Kun Yan 3 ; Moussian, Bernard 4 ; Li, Shen 1 ;

作者机构： 1.Shanxi Univ, Res Inst Appl Biol, Taiyuan 030006, Shanxi, Peoples R China

2.Shanxi Acad Agr Sci, Inst Plant Protect, Taiyuan 030031, Shanxi, Peoples R China

3.Kansas State Univ, Dept Entomol, 123 Waters Hall, Manhattan, KS 66506 USA

4.Univ Cote Azur, iBV, Parc Valrose, F-06000 Nice, France

5.South China Normal Univ, Guangzhou Key Lab Insect Dev Regulat & Applicat R, Inst Insect Sci & Technol, Guangzhou 510631, Guangdong, Peoples R China

6.South China Normal Univ, Sch Life Sci, Guangzhou 510631, Guangdong, Peoples R China

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN： 0965-1748

年卷期： 2018 年 101 卷

页码：

收录情况： SCI

摘要： Chitinases, key enzymes involved in degradation of chitin, have been repeatedly shown to play an indispensable role during insect post-embryonic molting processes at stage transitions. However, how chitinases affect insect embryonic development remains to be analyzed. In this study, we investigated the role of chitinase 5-1 (LmCht5-1) during embryonic development of the hemimetabolous insect Locusta migratoria. LmCht5-1 transcript levels were high in pro-nymphs during late embryogenesis. The respective protein localized to both the pro-nymphal and, to a much lesser extent, the newly formed nymphal cuticle. After injection of double stranded RNA against LmCht5-1 into 8 days old embryos, LmCht5-1 transcripts were strongly reduced. Most of dsLmCht5-1-injected pro-nymphs failed to develop to first-instar nymphs and died at or before hatching. Histological analyzes showed that degradation of the pro-nymph cuticle was blocked in these animals. At the ultrastructural level, we found that LmCht5-1 was needed for the degradation of the lamellar procuticle, while the separation of the procuticle from the epicuticle and epidermis (apolysis) was independent of LmCht5-1 function. Taken together, our results indicate that LmCht5-1 and other yet unknown degrading enzymes act in parallel at distinct positions of the cuticle during molting of the pro-nymph to the first-instar nymph during locust embryogenesis.

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