Isolation and Characterization of 19 Polymorphic Microsatellite Markers of Sepia esculenta
文献类型： 外文期刊
作者： Meng, Xiangbin 1 ; Gao, Tianxiang 2 ; Li, Chunhou 3 ; Song, Na 1 ; Liu, Lu 1 ; Liu, Liqin 2 ; Zhang, Xiumei 1 ;
作者机构： 1.Ocean Univ China, Fishery Coll, Qingdao, Peoples R China
2.Zhejiang Ocean Univ, Natl Engn Res Ctr Marine Aquaculture, Zhoushan 316004, Peoples R China
3.Chinese Acad Fishery Sci, Minist Agr, Key Lab Exploitat & Utilizat Marine Fisheries Res, Guangzhou, Guangdong, Peoples R China
4.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China
关键词： Sepia esculenta; Population structure; Genetic diversity; Polymorphic loci; Microsatellite makers
期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）
ISSN： 0030-9923
年卷期： 2018 年 50 卷 4 期
页码：
收录情况： SCI
摘要： Sepia esculenta is an economically important marine cephalopoda. The availability of highly polymorphic markers will be important to promote the conservation of this species. We performed next-generation sequencing and de novo assembly to obtain potential useful microsatellite markers for S. esculenta. Among 80 tested microsatellite markers, 19 showed polymorphism among S. esculenta individuals. The allele number of all polymorphic microsatellite markers ranged from 6 to 10. Expected and observed heterozygosity varied from 0.260 to 0.904 and from 0.292 to 0.861, with an average of 0.741 and 0.607, respectively. The polymorphism information content (PIC) ranged from 0.566 to 0.823. These markers will be useful in further studies and will give new insight into conservation and efficient management of this species.
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