Production and immobilization of lipase PCL and its application in synthesis of alpha-linolenic acid-rich diacylglycerol
文献类型： 外文期刊
作者： Liu, Nan 1 ; Li, Daoming 1 ; Wang, Weifei 2 ; Hollmann, Frank 3 ; Xu, Long 1 ; Ma, Yunjian 1 ; Yang, Bo 4 ; Bai, Weidong; 1 ;
作者机构： 1.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China
3.Delft Univ Technol, Dept Biotechnol, Van der Maasweg 9, NL-2629HZ Delft, Netherlands
4.South China Univ Technol, Sch Biosci & Bioengn, Guangzhou 510006, Guangdong, Peoples R China
5.Zhongkai Univ Agr & Engn, Coll Food Sci & Technol, Guangzhou 510225, Guangdong, Peoples R China
6.Beijing Technol & Business Univ, Beijing Key Lab Flavor Chem, Beijing 100048, Peoples R China
关键词： diacylglycerol; esterification; immobilization; reusability; alpha-Linolenic acid
期刊名称：JOURNAL OF FOOD BIOCHEMISTRY （ 影响因子：2.72； 五年影响因子：2.543 ）
ISSN： 0145-8884
年卷期： 2018 年 42 卷 5 期
页码：
收录情况： SCI
摘要： alpha-Linolenic acid-rich diacylglycerol has been demonstrated with promising health promotion functions. This study examined the production, immobilization of lipase PCL, and its application in the synthesis of diacylglycerol by esterification of -linolenic acid with glycerol. The resin ECR8806 was selected as an effective support for the immobilization of lipase PCL. Fourier transform infrared and Laser scanning confocal microscope analysis proved that the lipase was successfully immobilized on the resin. Compared with the free PCL, the immobilized one exhibited higher temperature tolerance. Under optimized reaction conditions, a DAG content of 54.49% were obtained. After further purified by molecular distillation, the purity of DAG was up to 99.28%. During esterification, the immobilized PCL was quite stable and retained more than 91.60% of its initial activity after 10 cycles. These new findings on the immobilized PCL will make it to be a prospective enzyme in oils and fats industry. Immobilized PCL, a monoacylglycerol and diacylglycerol lipase, was first employed to synthesis of a high purity -linolenic acid-rich diacylglycerol. The improved biocatalytic performance and reusability of the lipase make it to be a promising catalyst in industrial application.
- 相关文献
作者其他论文 更多>>
-
Combining phospholipase A1 with monoacylglycerol lipase for crude vegetable oil degumming through improved oil-water separation
作者：Liu, Xuan;Huang, Chunjin;Lan, Dongming;Wang, Weifei;Wang, Yonghua;Wang, Weifei;Wang, Yonghua
关键词：Crude vegetable oil; Enzymatic degumming; Oil-water separation; Phospholipids; Glycerophosphoesters; Phospholipase A1; Monoacylglycerol lipase
-
Volatile flavor and digestion behavior of peanut protein-based patties: Role of lipid type and oil phase incorporation method
作者：Chen, Ying;Zhang, Lin;Wang, Weifei
关键词：Plant-based patties; Acylglycerol; Volatile flavor; Digestion behavior
-
Comparative study of intelligent sensory technologies combined multiple metabolomics in evaluating sensory attributes and health profiles across different grades and varieties of green tea-processed mulberry leaf tea
作者：Lin, Junhui;Yang, Zhangchang;Li, Qian;Wang, Weifei;Liao, Sentai;Xing, Dongxu;Pang, Daorui;Zou, Yuxiao;Chen, Ruohong
关键词：Mulberry leaf tea; After frost; Different grades; Volatile metabolites; Nonvolatile metabolites; Bioactive substances; Lignans; Intelligent sensory technologies
-
Soy protein isolate-epigallocatechin-3-gallate non-covalent complex stabilized emulsion: Application as a fat phase in plant-based patty
作者：Chen, Ying;Zhang, Lin;Wang, Weifei
关键词：Meat analog; Epigallocatechin-3-gallate; Emulsion; Quality characteristic
-
Stability of probiotic microcapsules produced through complex coacervation based on whey protein-gum arabic coupled with double emulsification: Role of krill oil in middle oil phase
作者：Chen, Ying;Chen, Ying;Zhang, Weiqian;Wang, Weifei
关键词：Probiotic microcapsules; Double emulsification; Complex coacervation; Krill oil
-
Comparison of Dietary Inorganic and Small-Peptide Chelating Trace Minerals on Growth Performance, Immunity, Meat Quality, and Environmental Release in Litopenaeus vannamei
作者：Chen, Jingshen;Liu, Nan;Wang, Shumeng;Wang, Hailong;Ouyang, Kun;Wang, Yuxuan;Luo, Junyi;Sun, Jiajie;Xi, Qianyun;Zhang, Yongliang;Chen, Ting;Sun, Yuping;Si, Yongguo
关键词：complex small peptide; trace minerals; growth; immunity; meat quality; environmental release; Litopenaeus vannamei; complex small peptide; trace minerals; growth; immunity; meat quality; environmental release
-
How far are we from tailor-made human milk fat substitutes?
作者：Xu, Qingqing;Zhang, Fangyingnan;Zou, Qian;Wang, Yonghua;Wang, Weifei;Wang, Yonghua
关键词：Human milk fat substitutes; Infant formula; Tailor-made; Novel enzymatic synthesis; Geographical effect; Genetic polymorphisms
