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Production and immobilization of lipase PCL and its application in synthesis of alpha-linolenic acid-rich diacylglycerol

文献类型： 外文期刊

作者： Liu, Nan 1 ; Li, Daoming 1 ; Wang, Weifei 2 ; Hollmann, Frank 3 ; Xu, Long 1 ; Ma, Yunjian 1 ; Yang, Bo 4 ; Bai, Weidong; 1 ;

作者机构： 1.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China

3.Delft Univ Technol, Dept Biotechnol, Van der Maasweg 9, NL-2629HZ Delft, Netherlands

4.South China Univ Technol, Sch Biosci & Bioengn, Guangzhou 510006, Guangdong, Peoples R China

5.Zhongkai Univ Agr & Engn, Coll Food Sci & Technol, Guangzhou 510225, Guangdong, Peoples R China

6.Beijing Technol & Business Univ, Beijing Key Lab Flavor Chem, Beijing 100048, Peoples R China

关键词： diacylglycerol; esterification; immobilization; reusability; alpha-Linolenic acid

期刊名称：JOURNAL OF FOOD BIOCHEMISTRY （ 影响因子：2.72； 五年影响因子：2.543 ）

ISSN： 0145-8884

年卷期： 2018 年 42 卷 5 期

页码：

收录情况： SCI

摘要： alpha-Linolenic acid-rich diacylglycerol has been demonstrated with promising health promotion functions. This study examined the production, immobilization of lipase PCL, and its application in the synthesis of diacylglycerol by esterification of -linolenic acid with glycerol. The resin ECR8806 was selected as an effective support for the immobilization of lipase PCL. Fourier transform infrared and Laser scanning confocal microscope analysis proved that the lipase was successfully immobilized on the resin. Compared with the free PCL, the immobilized one exhibited higher temperature tolerance. Under optimized reaction conditions, a DAG content of 54.49% were obtained. After further purified by molecular distillation, the purity of DAG was up to 99.28%. During esterification, the immobilized PCL was quite stable and retained more than 91.60% of its initial activity after 10 cycles. These new findings on the immobilized PCL will make it to be a prospective enzyme in oils and fats industry. Immobilized PCL, a monoacylglycerol and diacylglycerol lipase, was first employed to synthesis of a high purity -linolenic acid-rich diacylglycerol. The improved biocatalytic performance and reusability of the lipase make it to be a promising catalyst in industrial application.

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