Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11
文献类型： 外文期刊
作者： Tang, Bao 1 ; Sun, Cheng 2 ; Zhao, Yancun 1 ; Xu, Huiyong 1 ; Xu, Gaoge 1 ; Liu, Fengquan 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China
2.Yangzhou Polytech Coll, Sch Resources & Environm Engn, Yangzhou 225009, Jiangsu, Peoples R China
关键词： Heat-stable antifungal factor; Lysobacter enzymogenes OH11; Biopesticides; Two-stage temperature control strategy
期刊名称：BMC BIOTECHNOLOGY （ 影响因子：2.563； 五年影响因子：3.292 ）
ISSN： 1472-6750
年卷期： 2018 年 18 卷
页码：
收录情况： SCI
摘要： BackgroundHeat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has reported on the fermentation process involved, representing huge obstacles for large-scale industrial production.ResultsMedium capacity, culture temperature, and fermentation time were identified as the most significant factors affecting the production of HSAF and employed for further optimization through statistical methods. Based on the analysis of kinetic parameters at different temperatures, a novel two-stage temperature control strategy was developed to improve HSAF production, in which the temperature was increased to 32 degrees C during the first 12h and then switched to 26 degrees C until the end of fermentation. Using this strategy, the maximum HSAF production reached 440.2616.14mgL(-1), increased by 9.93% than that of the best results from single-temperature fermentation. Moreover, the fermentation time was shortened from 58h to 54h, resulting in the enhancement of HSAF productivity (17.95%) and yield (9.93%).Conclusions p id= Par3 This study provides a simple and efficient method for producing HSAF that could be feasibly applied to the industrial-scale production of HSAF.
- 相关文献
作者其他论文 更多>>
-
OsPRMT5 methylates OsPAL1 to promote rice resistance, hindered by a Xanthomonas oryzae effector
作者：Sheng, Cong;Wang, Bo;Chen, Wenchan;Guo, Baodian;Zhao, Yancun;Liu, Fengquan;Li, Kaihuai;Liu, Fengquan;Wang, Bo;Chen, Wenchan;Qiao, Lulu;Zhao, Hongwei
关键词：effector; OsPAL1; OsPRMT5; protein arginine methylation; rice bacterial blight; SA
-
Sigma factor 70 RpoD contributes to virulence by regulating cell motility, oxidative stress tolerance, and manipulating the expression of hrpG and hrpX in Xanthomonas oryzae pv. oryzae
作者：Xu, Zhizhou;Liu, Fengquan;Liu, Fengquan;Xu, Zhizhou;Wang, Bo;Guo, Baodian;Sheng, Cong;Zhao, Yangyang;Tang, Bao;Zhao, Yancun;Liu, Fengquan;Wu, Guichun
关键词：Oryza sativa; sigma factor; pathogenicity; transcriptional regulation; type III secretion system
-
Apoplastic proteomic reveals Colletotrichum fructicola effector CfXyn11A recognized by tobacco and suppressed by pear in the apoplast
作者：Han, Chenyang;Tao, Shutian;Xie, Zhihua;Zhang, Shaoling;Liu, Fengquan;Liu, Fengquan
关键词：
Colletotrichum fructicola ; Pear; Apoplastic plant-microbe interaction; Fungal effector; CfXyn11A -
Lysobacter enzymogenes: A fully armed biocontrol warrior
作者：Lin, Long;Shao, Xiaolong;Yang, Yicheng;Murero, Aprodisia Kavutu;Wang, Limin;Han, Sen;Su, Zhenhe;Qian, Guoliang;Xu, Gaoge;Zhao, Yangyang;Xu, Kangwen;Liu, Fengquan;Han, Sen;Xu, Kangwen;Yang, Mingming;Liao, Jinxing;Li, Kaihuai;Liu, Fengquan
关键词：Lysobacter enzymogenes; antimicrobial metabolites; loss of flagella; type IV secretion system
-
Kasugamycin disrupts membrane transport and reduces virulence in Pectobacterium aroidearum to control konjac soft rot
作者：Xiong, Chunlan;Zhou, Xue;Zhu, Yu;Du, Xiaojuan;Li, Kaihuai;Liu, Fengquan;Xiong, Chunlan;Zhou, Xue;Zhu, Yu;Du, Xiaojuan;Li, Kaihuai;Liu, Fengquan;Tu, Yupei;Li, Kaihuai;Liu, Fengquan
关键词：Konjac; Soft rot;
Pectobacterium aroidearum ; Kasugamycin; Antibacterial activity -
First Report of Bacterial Fruit Blotch Caused by Kosakonia cowanii on Trichosanthis fructus in Jiangsu Province, China
作者：Chen, Xian;Tang, Bao;Zhao, Yancun;Liu, Fengquan;Chen, Xian;Tang, Bao;Laborda, Pedro
关键词：bacterial fruit blotch; Kosakonia cowanii; Trichosanthis fructus
-
Unveiling the Role of Diffusible Signal Factor-Family Quorum Sensing Signals in Regulating Behavior of Xanthomonas and Lysobacter
作者：Li, Kaihuai;Ma, Chaoyun;Xiong, Chunlan;Zhou, Xue;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Ma, Chaoyun;Xiong, Chunlan;Zhou, Xue;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Wang, Yong;Mao, Yahui;Liu, Fengquan
关键词：diffusible signal factor; Lysobacter enzymogenes; quorum sensing; Xanthomonas campestris
