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Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11

文献类型： 外文期刊

作者： Tang, Bao 1 ; Sun, Cheng 2 ; Zhao, Yancun 1 ; Xu, Huiyong 1 ; Xu, Gaoge 1 ; Liu, Fengquan 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

2.Yangzhou Polytech Coll, Sch Resources & Environm Engn, Yangzhou 225009, Jiangsu, Peoples R China

关键词： Heat-stable antifungal factor; Lysobacter enzymogenes OH11; Biopesticides; Two-stage temperature control strategy

期刊名称：BMC BIOTECHNOLOGY （ 影响因子：2.563； 五年影响因子：3.292 ）

ISSN： 1472-6750

年卷期： 2018 年 18 卷

页码：

收录情况： SCI

摘要： BackgroundHeat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has reported on the fermentation process involved, representing huge obstacles for large-scale industrial production.ResultsMedium capacity, culture temperature, and fermentation time were identified as the most significant factors affecting the production of HSAF and employed for further optimization through statistical methods. Based on the analysis of kinetic parameters at different temperatures, a novel two-stage temperature control strategy was developed to improve HSAF production, in which the temperature was increased to 32 degrees C during the first 12h and then switched to 26 degrees C until the end of fermentation. Using this strategy, the maximum HSAF production reached 440.2616.14mgL(-1), increased by 9.93% than that of the best results from single-temperature fermentation. Moreover, the fermentation time was shortened from 58h to 54h, resulting in the enhancement of HSAF productivity (17.95%) and yield (9.93%).Conclusions p id= Par3 This study provides a simple and efficient method for producing HSAF that could be feasibly applied to the industrial-scale production of HSAF.

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