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Uncovering the mechanisms underlying pear leaf apoplast protein-mediated resistance against Colletotrichum fructicola through transcriptome and proteome profiling

文献类型： 外文期刊

作者： Han, Chenyang 1 ; Su, Zhiyuan 1 ; Zhao, Yancun 2 ; Li, Chaohui 2 ; Guo, Baodian 2 ; Wang, Qi 1 ; Liu, Fengquan 2 ; Zhang, Shaoling 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Ctr Pear Engn Technol Res, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

2.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Inst Plant Protect,Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

关键词： Colletotrichum fructicola; Pear; Apoplast; Glycoside hydrolases; Plant immunity

期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.4； 五年影响因子：4.2 ）

ISSN： 2096-5362

年卷期： 2024 年 6 卷 1 期

页码：

收录情况： SCI

摘要： Pear anthracnose, caused by the fungus Colletotrichum fructicola, is a devastating disease for the pear industry. The apoplast, an extracellular compartment outside the plasma membrane, plays a crucial role in water and nutrient transport, as well as plant-microbe interactions. This study aimed to uncover the molecular mechanism of pear leaf apoplastic protein-mediated resistance to C. fructicola. Apoplast fluid was isolated using the vacuum infiltration method, and defence-related apoplastic proteins were identified through protein mass spectrometry and transcriptome sequencing. We found 213 apoplastic proteins in the leaf apoplast fluid during early C. fructicola infection, with the majority (74.64%) being enzymes, including glycosidases, proteases, and oxidoreductases. Gene Ontology analysis revealed their involvement in defence response, enzyme inhibition, carbohydrate metabolism, and phenylpropanoid biosynthesis. Transcriptome analysis showed the infection induced expression of certain apoplast proteins, potentially contributing to pear leaf resistance. Notably, the expression of PbrGlu1, an endo-beta-1,3-glucanase from the glycoside hydrolase 17 family, was significantly higher in infected leaves. Silencing of the PbrGlu1 gene increased pear leaf susceptibility to C. fructicola, leading to more severe symptoms and higher reactive oxygen species content. Overall, our study provides insights into the apoplast space interaction between pear leaves and C. fructicola, identifies a key gene in infected pears, and offers a foundation and new strategy for understanding the molecular mechanisms underlying pear anthracnose and breeding disease-resistant pears.

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