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Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line

文献类型： 外文期刊

作者： Liu, Qianchun 1 ; Wen, Changlong 1 ; Zhao, Hong 1 ; Wang, Yongqin 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Beijing 100097, Peoples R China

2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

关键词： Welsh onion; Cytoplasmic male sterility (CMS); atpA gene

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2019 年 243 卷

页码：

收录情况： SCI

摘要： Cytoplasmic male sterility (CMS) is a maternally inherited trait characterized by the inability to produce functional pollen and is important for exploiting heterosis in crop plants, but the molecular mechanisms of CMS and fertility restoration remain poorly understood in Welsh onion. Some CMS-associated genes were previously identified by RNA-Seq as candidate genes mediating male sterility in Welsh onion. In this study, the ATPase isoenzyme and ATPase activity were investigated. We also performed 2-dimensional electrophoresis to screen for ATPase-related proteins. The ATP synthase subunit alpha was found to be specifically expressed in the CMS line. Furthermore, we found that the full length of the atpA coding sequence was the same length in the CMS line and its maintainer line, but single nucleotide polymorphisms (SNPs) that lead to amino acid substitutions in the translated proteins were found. We hypothesize that the atpA gene is involved in regulating the development of CMS.

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