文献类型： 外文期刊

作者： Liu, Qing-Hao ¹ ; Guo, Jin-Chun ² ; Lu, Hong-Yu ¹ ; Guo, Yan-Nan ¹ ; Wang, Hai-Bin ¹ ; Hu, Zhi-Yong ¹ ; Liu, Hong-Yan; ¹ ;

作者机构： 1.North Univ China, Sch Chem Engn & Technol, Taiyuan 030051, Shanxi, Peoples R China

2.North Univ China, Sch Environm & Safety Engn, Taiyuan 030051, Shanxi, Peoples R China

3.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Henan, Peoples R China

4.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China

关键词： Oxazine dyes; Carboxyl groups; Good solubility; Staining method; PHAs-producing bacteria

期刊名称：JOURNAL OF FLUORESCENCE （ 影响因子：2.217； 五年影响因子：1.906 ）

ISSN： 1053-0509

年卷期： 2018 年 28 卷 6 期

页码：

收录情况： SCI

摘要： Derivatives of oxazine dyes were synthesized on mulitigram scales via efficient synthetic strategies. One practical route was selected to prepare compounds 6, 9 and 10, especially water-soluble compound 6 was obtained in better yield than reported, and compound 10 was insoluble in aqueous media in absence of phenolic-OH. Compounds 3 and 9 were found to be clear pH-dependent between pH=4.0 and 10.0, and could be used as acid-base indicators to measure intracellular pH. Compounds 6, 9, 10 all have carboxylic acid functionalities, which could be activated and used to conjugate the dyes to biomolecules. In addition, compounds 6 and 9 with good solubility in aqueous media were used to develop a simple, quick, safe, highly sensitive staining method to detect PHAs-producing bacteria on heat-fixed smears, which was confirmed by fluorescence images of PHAs granules of bacteria.