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Analysis of differential transcriptional profiling in wheat infected by Blumeria graminis f. sp tritici using GeneChip

文献类型： 外文期刊

作者： Wang, Jun-Mei 1 ; Liu, Hong-Yan 1 ; Xu, Hong-Ming 2 ; Li, Min 2 ; Kang, Zhen-Sheng 4 ;

作者机构： 1.Henan Key Lab Crop Pest Control, Zhengzhou 450002, Henan Province, Peoples R China

2.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Henan Province, Peoples R China

3.Minist Agr, IPM Key Lab So Part N China, Zhengzhou 450002, Henan Province, Peoples R China

4.NW A& F Univ, Plant Protect Coll, Yangling 712100, Shaanxi Provinc, Peoples R China

5.NW A& F Univ, Shanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi Provinc, Peoples R China

6.State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi Provinc, Peoples R China

关键词： Wheat;Powdery mildew;Transcriptional profiling;qRT-PCR (quantitative reverse transcription polymerase chain reaction)

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2012 年 39 卷 1 期

页码：

收录情况： SCI

摘要： Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease of wheat. The use of wheat cultivars resistant to powdery mildew provides an effective, economical, and environmentally friendly method to control the disease. Previously, we identified a dominant resistance gene, temporarily named Pmhym, from the wheat cultivar Hongyoumai. In order to screen differential transcripts related to Pmhym-mediated resistance, four F(3) homozygous resistant and four susceptible progenies derived from the Hongyoumai/Yumai13 cross were selected to construct two different pools, respectively, representing an incompatible and compatible interaction with Bgt. Pre-inoculated control and the pathogen-inoculated treatments at 24 h post inoculation (hpi) were used. Three groups of differential genes were categorized from three comparisons as pre- and post-induced, respectively, in two interactions, and post-induced between incompatible and compatible interaction. It was found that salicylic acid (SA), jasmonate (JA), and ethylene (ET) signaling-related genes were differentially expressed, thus suggesting that they are involved in the defensive response against Bgt infection. In compatible interactions, the genes involved in the abscisic acid (ABA) signaling pathway might be inhibitory to the above-mentioned three pathways, resulting in a susceptible reaction. Genes involved in disease/defense, signal transduction, and reactive oxygen species (ROS) metabolism were up-regulated in incompatible interactions, implying a role in resistant response. The results of qRT-PCR analysis on several candidate genes were consistent in their expression patterns as revealed by microarray analysis. The differential expression analyses in the present study are good candidates for further elucidation of wheat defensive response to powdery mildew.

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