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Biochemical and Functional Characterization of Anthocyanidin Reductase (ANR) from Mangifera indica L.

文献类型： 外文期刊

作者： Tan, Lin 1 ; Wang, Mei 2 ; Kang, Youfa 3 ; Azeem, Farrukh 1 ; Zhou, Zhaoxi 1 ; Tuo, Decai 5 ; Preciado Rojo, Lina Mari 1 ;

作者机构： 1.CATAS, Haikou Expt Stn, Hainan Key Lab Banana Genet Improvement, Haikou 570102, Hainan, Peoples R China

2.Univ Mississippi, Sch Pharm, Thad Cochran Res Ctr, Natl Ctr Nat Prod Res, University, MS 38677 USA

3.Hainan Univ, Dept Basic Educ Liberal Arts, Haikou 571101, Hainan, Peoples R China

4.Govt Coll Univ, Dept Bioinformat & Biotechnol, Faisalabad 38000, Pakistan

5.CATAS, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

6.Univ Antioquia, Fac Ciencias Farmaceut & Alimentarias, Programa Ofidismo Escorpionismo, Medellin 1226, Colombia

7.ARS, USDA, NPURU, POB 1848, University, MS 38677 USA

关键词： anthocyanidin reductase; (-)-epicatechin; (-)-catechin; proanthocyanidins; Mangifera indica L.

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2018 年 23 卷 11 期

页码：

收录情况： SCI

摘要： Mango (Mangifera indica L.) is abundant in proanthocyanidins (PAs) that are important for human health and plant response to abiotic stresses. However, the molecular mechanisms involved in PA biosynthesis still need to be elucidated. Anthocyanidin reductase (ANR) catalyzes a key step in PA biosynthesis. In this study, three ANR cDNAs (MiANR1-1,1-2,1-3) were isolated from mango, and expressed in Escherichia coli. In vitro enzyme assay showed MiANR proteins convert cyanidin to their corresponding flavan-3-ols, such as (-)-catechin and (-)-epicatechin. Despite high amino acid similarity, the recombinant ANR proteins exhibited differences in enzyme kinetics and cosubstrate preference. MiANR1-2 and MiANR1-3 have the same optimum pH of 4.0 in citrate buffer, while the optimum pH for MiANR1-1 is pH 3.0 in phosphate buffer. MiANR1-1 does not use either NADPH or NADH as co-substrate while MiANR1-2/1-3 use only NADPH as co-substrate. MiANR1-2 has the highest Km and Vmax for cyanidin, followed by MiANR1-3 and MiANR1-1. The overexpression of MiANRs in ban mutant reconstructed the biosynthetic pathway of PAs in the seed coat. These data demonstrate MiANRs can form the ANR pathway, leading to the formation of two types of isomeric flavan-3-ols and PAs in mango.

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