A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants
文献类型： 外文期刊
作者： Yan, Pu 1 ; Tuo, Decai 1 ; Shen, Wentao 1 ; Deng, Haida 1 ; Zhou, Peng 1 ; Gao, Xinzheng 3 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Sanya Res Inst, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Peoples R China
2.Hainan Inst Trop Agr Resources, Key Lab Biol & Genet Resources Trop Crops Hainan P, Haikou 571101, Peoples R China
3.Hainan Med Univ, Dept Biol, Haikou, Peoples R China
关键词： nimble cloning; plant expression vector; gene function; ectopic expression; genome editing
期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）
ISSN： 2590-3462
年卷期： 2023 年 4 卷 2 期
页码：
收录情况： SCI
摘要： Plant expression vectors are essential tools for gene functional analysis and molecular plant breeding. The gene of interest is transferred to the vector by molecular cloning technology. Nimble Cloning is a newly developed molecular cloning method with the advantages of simplicity, efficiency, and standardization. In this study, we developed a "pNC"vector system that contains 55 Nimble Cloning-compatible vectors for functional analysis of genes in plants. These vectors contain the NC frame flanked by unique adapters for one-step and standardized Nimble Cloning. We demonstrate that the pNC vectors are convenient and effective for the functional analysis of plant genes, including the study of gene ectopic expression, protein subcellular localization, protein-protein interaction, gene silencing (RNAi), virus-induced gene silencing, promoter activity, and CRISPR-Cas9-mediated genome editing. The "pNC"vector system represents a high-throughput toolkit that can facilitate the large-scale analysis of plant functional genomics.
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