Transcriptome analysis of Aeromonas hydrophila infected hybrid sturgeon (Huso dauricus x Acipenser schrenckii)
文献类型： 外文期刊
作者： Jiang, Nan 1 ; Fan, Yuding 1 ; Zhou, Yong 1 ; Wang, Weiling 1 ; Ma, Jie 1 ; Zeng, Lingbing 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Fish Dis Div, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2018 年 8 卷
页码：
收录情况： SCI
摘要： The hybrid sturgeon (Huso dauricus x Acipenser schrenckii) is an economically important species in China. With the increasing aquaculture of hybrid sturgeon, the bacterial diseases are a great concern of the industry. In this study, de novo sequencing was used to compare the difference in transcriptome in spleen of the infected and mock infected sturgeon with Aeromonas hydrophila. Among 187,244 unigenes obtained, 87,887 unigenes were annotated and 1,147 unigenes were associated with immune responses genes. Comparative expression analysis indicated that 2,723 differently expressed genes between the infected and mock-infected group were identified, including 1,420 up-regulated and 1,303 down-regulated genes. 283 differently expressed anti-bacterial immune related genes were scrutinized, including 168 up-regulated and 115 down-regulated genes. Ten of the differently expressed genes were further validated by qRT-PCR. In this study, toll like receptors (TLRs) pathway, NF-kappa B pathway, class A scavenger receptor pathway, phagocytosis pathway, mannose receptor pathway and complement pathway were shown to be up-regulated in Aeromonas hydrophila infected hybrid sturgeon. Additionally, 65,040 potential SSRs and 2,133,505 candidate SNPs were identified from the hybrid sturgeon spleen transcriptome. This study could provide an insight of host immune genes associated with bacterial infection in hybrid sturgeon.
- 相关文献
作者其他论文 更多>>
-
Cyprinid herpesvirus 2 ORF41 Protein Degrades Pyruvate Dehydrogenase (PDH)-E1β to Promote Viral Replication in Gibel Carp Brain (GiCB) Cells
作者：Xue, Mingyang;Xu, Chen;Huang, Zhenyu;Meng, Yan;Jiang, Nan;Fan, Yuding;Zhou, Yong;Xue, Mingyang
关键词：
Cyprinid herpesvirus 2 ; ORF41; pyruvate dehydrogenase -
Establishment and characterization of a hepatopancreas cell line from gibel carp (Carassius auratus gibelio) and its susceptibility to several fish viruses
作者：Duan, Qiudi;Ding, Huan;Ouyang, Lili;Ouyang, Shuang;Cao, Jiajia;Zhou, Jiezhi;Yuan, Ganjun;Wu, Xiaoyu;Pan, Xiaoyi;Li, Siqi;Tian, Yihan;Zhong, Qiwang;Fan, Yuding;Wu, Xiaoyu;Zhong, Qiwang
关键词：Hepatopancreas; Cell line; Chinese giant salamander iridovirus (GSIV); Spring viraemia of carp virus (SVCV); Gibel carp (Carassius auratus gibelio)
-
SHP-1 interacts with NFκB1 to inhibit its phosphorylation and nuclear translocation to exert its anti-bacterial function
作者：Wang, Ningning;Tan, Suxu;Wang, Muyuan;Liu, Hongning;Han, Sen;Wu, Zhendong;Ma, Jie;Chen, Songlin;Sha, Zhenxia;Wang, Ningning;Chen, Songlin;Chen, Songlin;Sha, Zhenxia
关键词：SHP-1; Immune response; Phosphorylation of NF kappa B1; Nuclear translocation
-
Identification of a novel circovirus associated with turbot (Scophthalmus maximus) acute hemorrhage disease
作者：Wang, Huilin;Huang, Zhihui;Li, Jie;Jiang, Jingzhe;Xu, Liming;Zhou, Yong;Qin, Qiwei;Wei, Jingguang;Wang, Qiyao;Xiao, Zhizhong;Li, Jie
关键词：Turbot; Turbot acute hemorrhage disease; Circovirus; Pathogen
-
Antiviral activity of arctigenin against Chinese rice-field eel rhabdovirus in Monopterus albus
作者：Liu, Yisha;Xue, Mingyang;Xu, Chen;Zhou, Yong;Jiang, Nan;Meng, Yan;Li, Yiqun;Huang, Zhenyu;Liu, Wenzhi;Li, Zhong;Fan, Yuding;Liu, Yisha;Zhong, Qiwang
关键词：Arctigenin; Rhabdovirus; Monopterus albus; Antiviral
-
Viperin and Its Effect on SVCV Replication in Common Carp, Cyprinus carpio
作者：Meng, Yan;Hu, Xi;Jiang, Nan;Fan, Yuding;Li, Yiqun;Xue, Mingyang;Xu, Chen;Liu, Wenzhi;Zhou, Yong
关键词：common carp (
Cyprinus carpio );viperin ; characteristics; antiviral activities -
Immunological Responses and Protection in the Largemouth Bass (Microterus salmoides) Immunized with Inactivated Vaccine Against Largemouth Bass Ranavirus (LMBRaV)
作者：Yang, Tao;Zhai, Jiale;Zeng, Lingbing;Zhou, Yong;Yang, Tao;Li, Chenyang;Yang, Tao;Zhai, Jiale;Zeng, Lingbing;Li, Yiqun;Liu, Wenzhi;Meng, Yan;Fan, Yuding;Huang, Zhenyu;Zhou, Yong;Jiang, Nan
关键词：largemouth bass; largemouth bass ranavirus; inactivated vaccine; relative percent survival rates; immune response
