您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Virulence regulation of cel-EIIB protein mediated PTS system in Streptococcus agalactiae in Nile tilapia

文献类型： 外文期刊

作者： Xu, Jun 1 ; Xie, Yun-Dan 3 ; Liu, Ling 1 ; Guo, Song 1 ; Su, You-Lu 2 ; Li, An-Xing 1 ;

作者机构： 1.Sun Yat Sen Univ, State Key Lab Biocontrol, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr, Key Lab South China Sea Fishery Resources Exploit, Guangzhou, Guangdong, Peoples R China

3.Tianjin Agr Univ, Coll Fisheries, Tianjin, Peoples R China

关键词： biofilm formation; cel-EIIB protein; colonization; Streptococcus agalactiae; virulence regulation

期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）

ISSN： 0140-7775

年卷期： 2019 年 42 卷 1 期

页码：

收录情况： SCI

摘要： Streptococcus agalactiae is a major pathogen of tilapia causing significant economic losses for the global aquatic industry yearly. To elucidate the role of cel-EIIB protein-mediated phosphotransferase systems (PTS) in the virulence regulation of S. agalactiae, cel-EIIB gene deletion in a virulent strain THN0901 was achieved by homologous recombination. The cellobiose utilization of ocel-EIIB strain was significantly decreased relative to S.a.THN0901 strain incubating in LB with 10 mg/ml cellobiose (p < 0.05). The biofilm formation ability of ocel-EIIB strain was also significantly decreased when cultured in BHI medium (p < 0.05). Under a lower infection dose, the accumulative mortality of tilapia caused by ocel-EIIB strain was dramatically decreased (20%), of which S.a.THN0901 strain and ocel-EIIB::i strain were 53.33% and 50%, respectively. The competition experience using tilapia model indicated the invasion and colonization ability of ocel-EIIB strain was significantly weaker than that of S.a.THN0901 strain (p < 0.05). Compared to ocel-EIIB::i strain, the mRNA expression of csrS, csrR, rgfA, rgfC, bgrR and bgrS was significantly downregulated in ocel-EIIB strain (p < 0.05). In conclusion, cel-EIIB protein-mediated cel-PTS not only contributes to biofilm formation and virulence regulation, but also plays an important role in the invasion and colonization of S. agalactiae.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台