Delignification overmatches hemicellulose removal for improving hydrolysis of wheat straw using the enzyme cocktail from Aspergillus niger
文献类型： 外文期刊
作者： Wang, Jiahong 1 ; Chen, Xuntong 2 ; Chio, Chonlong 2 ; Yang, Chun 2 ; Su, Erzheng 1 ; Jin, Yongcan 1 ; Cao, Fuliang 3 ; Q 1 ;
作者机构： 1.Nanjing Forestry Univ, Coll Light Ind & Food Engn, Dept Food Sci & Technol, Nanjing 210037, Jiangsu, Peoples R China
2.Lakehead Univ, Dept Biol, Thunder Bay, ON P7B 5E1, Canada
3.Nanjing Forestry Univ, Coll Forestry, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Jiangsu, Peoples R China
4.Shanxi Acad Agr Sci, Inst Agr Prod Proc, Tianyuan 030031, Peoples R China
关键词： Wheat straw; Aspergillus niger; Xylanase; Enzymatic hydrolysis; Delignification; Hemicellulose removal
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）
ISSN： 0960-8524
年卷期： 2019 年 274 卷
页码：
收录情况： SCI
摘要： Based on a general understanding that hemicellulose removal is more efficient than delignification for biomass deconstruction, an Aspergillus niger strain producing high xylanase activity was screened out from seventeen strains by clear halo experiments. Low-cost enzyme cocktail with high xylanase activity was produced from wheat straw medium fermented by the Gyx086 strain. The enzyme cocktail with high xylanase activity could more effectively hydrolyze wheat straw than other biomasses. However, only 30% of total carbohydrates could be hydrolyzed to reducing sugar in untreated wheat straw. Further enzymatic hydrolysis and pretreated trials were carried out, the results indicated that hemicellulose removal was less effective than delignification for de-recalcitrance of wheat straw and the crystallinity is little interference with the hydrolysis process. Delignified wheat straw was near-completely hydrolyzed by the enzyme cocktail in 60 h. This study advanced the knowledge in promoting wheat straw as feedstock for bio-based industry.
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