Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses
文献类型： 外文期刊
作者： Lin, Su 1 ; Wang, Shao 1 ; Cheng, Xiaoxia 1 ; Xiao, Shifeng 1 ; Chen, Xiuqin 1 ; Chen, Shilong 1 ; Chen, Shaoying 1 ; Yu, 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350003, Fujian, Peoples R China
2.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou 350003, Fujian, Peoples R China
3.Fujian Anim Dis Control Technol Dev Ctr, Fuzhou 350013, Fujian, Peoples R China
关键词： GPV; MDPV; SYBR Green I; Duplex real-time PCR; Parvovirus
期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）
ISSN： 1743-422X
年卷期： 2019 年 16 卷
页码：
收录情况： SCI
摘要： BackgroundWaterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important.ResultsA duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm=87.30.26 degrees C or Tm=85.4 +/- 0.23 degrees C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded.Conclusion This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections.
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