文献类型： 外文期刊

作者： Wang Si-Wei ¹ ; Liu Yan-Ping ¹ ; Sun Hai-Bin ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Plant Protect, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Guangdong, Peoples R China

关键词： Ultra performance liquid chromatography-tandem mass spectrometry; Pyraclostrobin; Metabolite; Pollen; Honey

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2018 年 46 卷 5 期

页码：

收录情况： SCI

摘要： An effective method was developed for determination of pyraclostrobin and BF 500-3 residues in pollen and honey of litchi by QuEChERS coupled with ultra performance liquid chromatography. tandem mass spectrometry. The samples were extracted by acetonitrile and cleaned by primary secondary amine (PSA) and C-18. In the positive ion mode and multi reaction monitoring mode, the analytes were quantified by the matrix matching standard solutions, and the pretreatment and mass spectrometry conditions were evaluated. The matrix matched standard solutions of pyraclostrobin and its metabolite BF 500-3 showed good linearities in the concentration range of 1-100 mu g/L, with the correlation coefficients (R-2) of 0. 991-0. 999. The average recoveries were 87. 0%-97. 8% with relative standard deviations of 1. 3%-3. 7%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0. 08-0. 20 mu g/kg and 0. 20-0. 50 mu g/kg, respectively. The method was easy, quick and highly sensitive, and was suitable for quick determination of pyraclotrobin and its metabolites in pollen and honey of litchi.