RNA-Seq analysis of gynoecious and weak female cucumber revealing the cell cycle pathway may regulate sex determination in cucumber
文献类型： 外文期刊
作者： Wang, Rui 1 ; Lin, Yu'e 1 ; Jin, Qingmin 1 ; Yao, Chunpeng 1 ; Zhong, Yujuan 1 ; Wu, Tingquan 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Guangdong, Peoples R China
2.Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Guangdong, Peoples R China
关键词： RNA-Seq; Gynoecious cucumber; Cell cycle; Sex determination
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
ISSN： 0378-1119
年卷期： 2019 年 687 卷
页码：
收录情况： SCI
摘要： This study aims to investigate key genes involved in molecular regulatory networks of cucumber sex determination. Genome-wide high-throughput RNA sequencing was performed for young apical buds of gynoecious and weak female cucumber at three growth stages (one-leaf one-bud, three-leaf one-bud, and five-leaf one-bud). Seven comparisons from the same cultivar at three different stages and at the same stage between the two cultivars were analyzed, and the results revealed that compared with differentially expressed genes (DEGs) in weak female cucumber, more genes were upregulated at the one-leaf one-bud stage and downregulated at the three-leaf one-bud stage in gynoecious cucumber. In addition, there were four kinds of gene expression trends (0, 1, 6, and 7), which were significantly enriched in gynoecious cucumber, while only two kinds of gene expression trends (5 and 6) were significantly enriched in weak female cucumber. Together with the data of the Gene Ontology (GO), pathway, gene expression trends and qRT-PCR, nine genes were identified and considered as candidate genes that may be involved in sex differentiation regulation in cucumber. These genes included Cs-MCM6, Cs-ACTS, Cs-XRCC4, Cs-MCM2, Cs-CDC45, Cs-Dpri, Cs-H2B, Cs-CDC20 and Cs-CNGC1. Among these genes, five genes (Cs-MCM6, Cs-MCM2, Cs-CDC45, Cs-Dpri, and Cs-CDC20) were involved in the cell cycle pathway, suggesting that the cell cycle pathway may play an important role in sex determination in cucumber.
- 相关文献
作者其他论文 更多>>
-
Transcriptomic analysis reveals the molecular basis of photoperiod-regulated sex differentiation in tropical pumpkins (Cucurbita moschata Duch.)
作者：Xue, Shudan;Huang, Hexun;Xu, Yingchao;Liu, Ling;Meng, Qitao;Zhu, Jitong;Zhou, Meijiang;Du, Hu;Yao, Chunpeng;Jin, Qingmin;Zhong, Yujuan;Meng, Qitao;Nie, Chengrong
关键词：Photoperiod; Cucurbita moschata; Sex differentiation; Photoperiod-mediated flowering processes; Gibberellin signaling pathway; Ethylene biosynthetic and ethylene response pathways
-
Excavation of Genes Response to Heat Resistance by Transcriptome Analysis in Bottle Gourd (Lagenaria siceraria (Mol.) Standl.)
作者：Wang, Min;Liu, Wenrui;Peng, Qingwu;Shi, Shaoqi;Jiang, Biao;Lin, Yu'e;Yang, Songguang;Wang, Min;Liu, Wenrui;Shi, Shaoqi;Cao, Liqin;Jiang, Biao;Zhao, Tianyue;Cui, Xiaojuan;Yang, Songguang;Wang, Ying
关键词：bottle gourd; RNA-Seq; DEGs; MAPK; bHLH; heat stress
-
Identification of the G2-like transcriptional factor gene family reveals their critical roles in controlling chlorophyll accumulation of melon fruit
作者：Cao, Haishun;Wu, Tingquan;Zhang, Changyuan;Shi, Liangliang;Gong, Mingxi;Anwar, Ali;Song, Shiwei
关键词：G2-like transcription factors; Melon; Chlorophyll; Fruit peel; GLKs; APRR2
-
Rapid Construction and Application of a Vector for Tobacco Ringspot Virus-Induced McPDS Silencing in Bitter Gourd
作者：Zeng, Lingen;Deng, Zhijun;Zeng, Lingen;Zhang, Hui;Liu, Hongbiao;Du, Hu;Zhong, Yujuan;Yao, Chunpeng;Zhang, Hui;Guo, Jinju;Zhang, Changyuan
关键词：bitter gourd; virus-induced gene silencing; TRSV; phytoene dehydrogenase
-
Genome-wide identification and expression analysis of the cryptochromes reveal the CsCRY1 role under low-light-stress in cucumber
作者：Cao, Haishun;Wang, Rui;Zhao, Junhong;Shi, Liangliang;Wu, Tingquan;Zhang, Changyuan;Huang, Yuan
关键词：cryptochromes; cucumber; low-light-stress; photoreceptors; alternative splicing
-
Potential locus W and candidate gene McPRR2 associated with pericarp pigment accumulation in bitter gourd (Momordica charantia L.) revealed via BSA-seq analysis
作者：Guo, Jinju;Han, Xin;Wu, Tingquan;Wang, Rui;Zhao, Junhong;Wang, Rufang;Tan, Delong;Zhang, Huiyao;Zhang, Changyuan;Yan, Shijuan;Huang, Wenjie;Gao, Jie
关键词：Bitter gourd; BSA-seq; McPRR2; Chlorophyll; Pericarp color
-
Identification of candidate genes controlling cucumber hypocotyl elongation under low light stress based on BSA-seq and RNA-seq
作者：Chen, Lin;Meng, Haoyuan;Chen, Baoying;Xie, Shuyan;Liu, Wenrui;Wang, Min;Yan, Jinqiang;Cai, Jinsen;Yang, Songguang;Jiang, Biao;Peng, Qingwu;Lin, Yu'e
关键词：Cucumber; Hypocotyl; Low light stress; BSA-seq; RNA-seq
