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Excavation of Genes Response to Heat Resistance by Transcriptome Analysis in Bottle Gourd (Lagenaria siceraria (Mol.) Standl.)

文献类型： 外文期刊

作者： Wang, Min 1 ; Liu, Wenrui 1 ; Peng, Qingwu 1 ; Shi, Shaoqi 1 ; Wang, Ying 3 ; Cao, Liqin 2 ; Jiang, Biao 1 ; Lin, Yu'e 1 ; Zhao, Tianyue 2 ; Cui, Xiaojuan 2 ; Yang, Songguang 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Peoples R China

2.Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310012, Peoples R China

关键词： bottle gourd; RNA-Seq; DEGs; MAPK; bHLH; heat stress

期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN：

年卷期： 2024 年 14 卷 2 期

页码：

收录情况： SCI

摘要： Heat stress, as a negative factor, severely threatens the quality and production of bottle gourd, which prefers to grow in a warm environment. To understand which genes are involved in the resistance to heat stress in bottle gourd (Lagenaria siceraria (Mol.) Standl.), we analyzed the characteristics of two genetic bottle gourd varieties, "Mei feng"-MF (heat resistant) and "Lv long"-LL (heat sensitive). Under heat stress, MF plants exhibited a higher survival rate, lower relative electrolytic leakage, and decreased stomatal aperture compared with LL. In addition, RNA-Seq was carried out on the two varieties under normal conditions and heat stress. The results revealed a total of 1485 up-regulated and 946 down-regulated genes under normal conditions, while 602 genes were up-regulated and 1212 genes were down-regulated under heat stress. Among these genes, several differentially expressed genes (DEGs) involved in the MAPK (mitogen-activated protein kinase) signaling pathway and members of bHLH (basic helix-loop-helix) transcription factors showed significant up- or down-regulation after heat stress. Next, to validate these findings, we conducted quantitative real-time PCR (qRT-PCR) analysis, which confirmed the expression patterns of the genes detected through RNA-Seq. Collectively, the DEGs between the two contrasting cultivars identified in our study provide novel insight into excavating helpful candidate genes associated with heat tolerance in bottle gourd.

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