Cloning and functional analysis of squalene synthase gene from Dryopteris fragrans (L.) Schott
文献类型： 外文期刊
作者： Gao, Rui 1 ; Yu, Dan 2 ; Chen, Lingling 1 ; Wan, Wenzong 3 ; Sun, Lili 4 ; Chan, Ying 1 ;
作者机构： 1.Northeast Agr Univ, Coll Life Sci, Lab Plant Res, Harbin 150030, Heilongjiang, Peoples R China
2.Northeast Agr Univ, Coll Life Sci, Biopharmaceut Lab, Harbin 150030, Heilongjiang, Peoples R China
3.Heilongjiang Acad Agr Sci, Virus Free Seedling Res Inst, Harbin 150086, Heilongjiang, Peoples R China
4.Heilongjiang Acad Agr Sci, Hort Branch, Harbin 150069, Heilongjiang, Peoples R China
关键词： Dryopteris fragrans (L.) Schott; Squalene synthase; Triterpenoids
期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.65； 五年影响因子：1.548 ）
ISSN： 1046-5928
年卷期： 2019 年 155 卷
页码：
收录情况： SCI
摘要： Dryopteris fragrans (L.) Schott is a traditional herbal medicine containing medicinal sterols and triterpenoids. Squalene synthase (SQS) is the first crucial enzyme in the biosynthesis pathway of sterols and triterpenoids. The full-length cDNA named DfSQS1 was isolated by RACE. It was predicted that DfSQS1 contained an open reading frame (ORF) of 1239 bp coding 412 amino acid residues with molecular weight of 46.6 kDa. It had 18 potential phosphorylation sites, 1 potential N-glycosylation site and 2 transmembrane domains. In neighbor-joining (NJ) phylogenetic tree, DfSQS1 was away from branch of gymnosperms and angiosperms. One hydrophobic domain at the C-terminal of DfSQS1 was deleted to express soluble recombinant enzyme. The truncated DfSQS1 (tDfSQS1) was expressed in Escherichia coli BL21 (DE3). Then, tDfSQS1 was obtained and incubated with famesyl diphosphate (FPP) to identify its enzymatic activity. The result demontrated that squalene, the product of enzyme catalyzed reaction, was detected by HPLC. Quantitative real-time PCR (qRT-PCR) analysis revealed that the transcription level of DfSQS1 in D. fragrans was the highest in roots, followed by leaves and rhizomes. This work is the first report on cloning, characteration and expression of SQS from D. fragrans. It will be helpful to understand the regulatory role of SQS on the biosynthesis of triterpenoids in the fern.
- 相关文献
作者其他论文 更多>>
-
Transcriptome Profile Analysis of Resistance Inoculated byDryopteris crassirhizomaDryocrassin ABBA in Potato Tubers
作者：Wang, Wenzhong;Min, Fanxiang;Yang, Shuai;Wei, Qi;Guo, Mei;Wang, Wenzhong;Gao, Rui;Zhang, Tong;Chen, Lingling;Feng, Peng;Zhao, Zongbao;Zhang, Changxu;Chang, Ying;Gao, Rui
关键词：Dryopteris crassirhizomadryocrassin ABBA; Induced resistance; Transcriptome; Solanum tuberosum; Molecular mechanism
-
CLONING AND EXPRESSION OF CINNAMATE 4-HYDROXYLASE GENE FROM DRYOPTERIS FRAGRANS (L.) SCHOTT
作者：Sun, Lili;Zhang, Liguo;Zhang, Xiaofei;Chen, Lingling;Bu, Zhigang;Chang, Ying;Wang, Fude;Mu, Yunhui;Chen, Lixin;Guo, Li;Zhang, Xiaofei;Chen, Lingling;Chang, Ying;Wang, Fude;Bu, Zhigang
关键词：Gene clone; Dryopteris fragrans; Transformation; Cinnamate 4-hydroxylase; Overexpression
-
OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis
作者：Zhang, Liguo;Sun, Lili;Zhang, Shuquan;Xie, Dongwei;Liang, Chunbo;Huang, Wengong;Fan, Lijuan;Fang, Yuyan;Zhang, Liguo;Chang, Ying;Zhang, Xiaofei
关键词：OFP1; ATH1; interaction; stem elongation; flowering; boundary
-
Complete chloroplast genome sequence of Dryopteris fragrans (L.) Schott and the repeat structures against the thermal environment
作者：Gao, Rui;Wang, Wenzhong;Wang, Xu;Feng, Peng;Zhao, Guangming;Bian, Shuang;Ren, Hongli;Chang, Ying;Wang, Wenzhong;Huang, Qingyang;Fan, Ruifeng
关键词：
-
DE NOVO TRANSCRIPTOME SEQUENCEING AND COMPARATIVE ANALYSIS OF DIFFERENTIALLY EXPRESSED GENES IN DRYOPERIS FRAGRANS UNDER TEMPERATURE STRESS
作者：Wang, Wen-Zhong;Tong, Wei-Shuang;Li, Yan;Gao, Rui;Zhang, Liguo;Chang, Ying;Wang, Wen-Zhong;Zhang, Liguo
关键词：Transcriptome;Dryopteris fragrans;Flavonoids biosynthesis;DEG (differentially expressed genes)
-
Dryopteris crassirhizoma Dryocrassin ABBA for Postharvest Control of the Potato Dry Rot Pathogen Fusarium solani var. coeruleum
作者：Wang, Wenzhong;Gao, Rui;Bo, Zhigang;Chen, Lingling;Chang, Ying;Wang, Wenzhong;Min, Fanxiang;Gao, Yunfei;Yang, Shuai;Wei, Qi;Dong, Xuezhi;Lv, Dianqiu
关键词：Dryopteris crassirhizoma dryocrassin ABBA;Fusarium solani var;coeruleum;induced resistance;potato dry rot
