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The evaluation of an immunoperoxidase assay applicable in antiviral drug screening

文献类型： 外文期刊

作者： Zhu, Liqian 1 ; Huang, Liai 1 ; Wang, Aiping; Li, Qingmei 1 ; Guo, Junqing 1 ; Wang, Li 1 ; Zhang, Gaiping 1 ;

作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

2.Yangzhou Univ, Coll Vet Med, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

3.Yang

关键词： BVDV; NCP-BVDV; Antiviral drug; inhibitor

期刊名称：BIOLOGICALS （ 影响因子：1.856； 五年影响因子：2.05 ）

ISSN： 1045-1056

年卷期： 2019 年 57 卷

页码：

收录情况： SCI

摘要： Bovine viral diarrhea virus (BVDV) fall into cytopathic (CP) and noncytopathic (NCP) biotypes, based on their ability to kill cultured cells. NCP-BVDV can not be titrated by conventional means as used for CP-BVDV, which has impeded the identification of antiviral drugs targeting NCP-BVDV virus strains. In this study, the application of an immunoperoxidase assay in the screening of antiviral drugs was tested using two known BVDV inhibitors, ribavirin and ammonium chloride (NH4CI). Phospholipase C inhibitor U73122 was identified to affect BVDV infection by using this immunoperoxidase assay. in addition, the results of immunoperoxidase assay were validated by real-time PCR. Taken together, the immunoperoxidase assay is a useful and versatile method suitable for antiviral drug screening targeting NCP-BVDV.

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