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Functional characterization of a matrix metalloproteinase 2 gene in Litopenaeus vannamei

文献类型： 外文期刊

作者： Lian, Yu-Ying 1 ; He, Hong-Hui 2 ; Zhang, Chao-Zheng 5 ; Li, Xin-cang 6 ; Chen, Yi-Hong 7 ;

作者机构： 1.Sun Yat Sen Univ, Key Lab Marine Resources & Coastal Engn Guangdong, South China Sea Bioresource Exploitat & Protect C, Sch Marine Sci, 135 Xingang Rd West, Guangzhou 510275, Guangdong, Peoples R China

2.Sun Yat Sen Univ, Sch Life Sci, 135 Xingang Rd West, Guangzhou 510275, Guangdong, Peoples R China

3.Sun Yat Sen Univ, State Key Lab Biocontro, Inst Aquat Econ Anim, 135 Xingang Rd West, Guangzhou 510275, Guangdong, Peoples R China

4.Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, 135 Xingang Rd West, Guangzhou 510275, Guangdong, Peoples R China

5.Guangdong Prov Ctr Dis Control & Prevent, 160 QunXian Rd, Guangzhou 511430, Guangdong, Peoples R China

6.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

7.South Chi

关键词： Matrix metalloproteinase 2; Oxidative stress; Antimicrobial peptide gene; Litopenaeus vannamei

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2019 年 84 卷

页码：

收录情况： SCI

摘要： Matrix metalloproteinases (MMPs) contribute to both normal and pathological tissue remodeling. They act as regulatory molecules by working in enzyme cascades as well as processing matrix proteins, cytokines, growth factors and adhesion molecules to generate fragments with biological effects. So MMPs could play distrinct roles in the process of pathogen infection. In present study, we cloned a MMP-2 (LvMMP-2) gene from Litopenaeus vannamei. LvMMP-2, highly expressed in epidermis, located to endoplasmic reticulum in S2 cells. Results of realtime RT-PCR assay showed that LvMMP-2 was induced in shrimp hemocytes upon unfolded protein response or oxidative stress, but not via heat shock treatment. It is proved that the promoter activity of LvMMP-2 was enhanced by NF-E2-related factor 2 and AP-1 factor c-Jun. Further research showed that down-regulated LvMMP-2 contributing to oxidative stress injury, could reduce the cumulative mortality of shrimps under oxidative stress. Besides, our study also indicated that LvMMP-2 was accelerated by lipopolysaccharides injection. LvMMP-2 in S2 could increase the promoter activity of several antimicrobial peptide genes, and knocked-down expression of LvMMP-2 depressed the expression of penaddin2 and beta-Defensin. Moreover, we showed that down regulated LvMMP-2 suppressed the cumulative mortality of shrimp infected with white spot syndrome virus (WSSV) or with Vibrio atinolyticus. Collecting results suggested that LvMMP-2 involves in shrimp innate immune response, and also contributes to tissue injury caused by WSSV infection.

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