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Molecular cloning, tissue distribution, and pharmacological characterization of blunt snout bream (Megalobrama amblycephala) melanocortin-5 receptor

文献类型： 外文期刊

作者： Liao, Shengchen 1 ; Dong, Jingjing 1 ; Xu, Weina; Xi, Bingwen 1 ; Tao, Yaxiong; Liu, Bo 1 ; Xie, Jun 1 ;

作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Peoples R China

3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwa

关键词： Megalobrama amblycephala; MC5R; Gene cloning; Tissue expression; Pharmacological characterization

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2019 年 45 卷 1 期

页码：

收录情况： SCI

摘要： The melanocortin-5 receptor (MC5R) plays an important role in the regulation of exocrine secretion in mammals. Its function in fish is not well established. In this study, we reported the molecular cloning, tissue expression, and pharmacological characterization of Megalobrama amblycephala MC5R (MamMC5R), as well as the effect of catching stress on its expression. The full-length cDNA of Mammc5r gene was 1237bp, consisted of a 990-bp open reading frame encoding 329 amino acids. Sequence analyses revealed that the nucleotide and amino acid sequences of Mammc5r were highly homologous (>90%) with MC5Rs of zebrafish, common carp, and goldfish. Tissue expression profile analysis showed that Mammc5r was widely expressed in both central and peripheral tissues, with the highest expression in the brain. Catching stress significantly changed the expression of Mammc5r in the skin, brain, and eye. In the skin, the expression level of Mammc5r was significantly reduced at 1h and 4h and increased at 24h after catching stress. The Mammc5r expression level was rapidly upregulated in the brain and eye at 1h and then decreased to the level before stress at 4h and 24h. With human MC5R (HsaMC5R) as a control, several agonists including -melanocyte-stimulating hormone (-MSH), and -MSH in addition to an analogue [Nle(4), D-Phe(7)]--MSH (NDP-MSH), were used to investigate the binding and signaling properties of MamMC5R. The results revealed that MamMC5R had the highest affinity for NDP-MSH, followed by - and -MSH. Taken together, the data suggested that MamMC5R might play a role in stress response in M. amblycephala.

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