Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal Mushroom Inonotus obliquus
文献类型： 外文期刊
作者： Liu, Zhendong 1 ; Yu, Dongsheng 2 ; Li, Liang 1 ; Liu, Xiaoxiao 2 ; Zhang, Henan 2 ; Sun, Wenbo 5 ; Lin, Chi-Chung 2 ; Ch 1 ;
作者机构： 1.Tibet Agr & Anim Husb Univ, Food Sci Coll, Linzhi 860000, Peoples R China
2.Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Shanghai Acad Agr Sci, Inst Edible Fungi, Beijing, Peoples R China
3.Natl R&D Ctr Edible Fungi Proc, Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China
4.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China
5.Shandong Acad Agr Sci, Anim Sci & Vet Med Inst, Shandong Prov Key Lab Anim Dis Control & Breeding, Jinan 250100, Shandong, Peoples R China
关键词： Inonotus obliquus; three-phase partitioning; free-radical scavenging abilities; antioxidant activity; immunological activity
期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）
ISSN： 1420-3049
年卷期： 2019 年 24 卷 3 期
页码：
收录情况： SCI
摘要： Polysaccharides from the immunomodulatory medicinal mushroom Inonotus obliquus (IOPS) were extracted and purified using three-phase partitioning (TPP), which is an efficient, fast, safe, and green purification technique. An optimal extraction procedure that gave a good 2.2% isolated yield was identified, using the following protocol: a solid-liquid ratio of 1 g to 12 mL; mass fraction of (NH4)(2)SO4 20% (w/v); 11 mL t-butanol; pH 8.0; temperature 30 degrees C; and extraction time 30 min. The purified IOPS was shown to be a proteoglycan of 40 kDa molecular weight comprising of d-galactose, d-glucose, d-xylose, and d-mannose in a molar ratio of 2.0:3.5:1.0:1.5. The purified IOPS displayed strong free-radical scavenging abilities, antioxidant activities, and immunological activity in vitro. IOPS' Trolox antioxidant equivalent capacity and ferric-reducing ability of plasma were 251.2 mu mol Trolox/g sample and 1040.5 mu mol Fe2+/g sample, respectively, with the activity of its immunomodulatory behavior shown to be gradient dependent.
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