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miR-10b-5p Regulates C2C12 Myoblasts Proliferation and Differentiation

文献类型： 外文期刊

作者： Ge, Guihua 1 ; Yang, Dongli 2 ; Tan, Ya 1 ; Chen, Ying 1 ; Jiang, Dongmei 1 ; Jiang, Anan 1 ; Li, Qiang 4 ; Liu, Yihui 4 ; Z 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Anim Sci & Technol, Chengdu, Sichuan, Peoples R China

2.Luzhou Anim Husb Stn, Luzhou, Sichuan, Peoples R China

3.Guizhou Acad Agr Sci, Inst Anim Husb & Vet, Guiyang, Guizhou, Peoples R China

4.Sichuan Prov Gen Stn Anim Husb, Chengdu, Sichuan, Peoples R China

5.Sichuan Anim Sci Acad, Anim Breeding & Genet Key Lab Sichuan Prov, Chengdu, Sichuan, Peoples R China

关键词： Mir-10b-5p; NFAT5; myogenesis; C2C12 myoblasts

期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）

ISSN： 0916-8451

年卷期： 2019 年 83 卷 2 期

页码：

收录情况： SCI

摘要： The development of skeletal muscle is a complex process including myoblasts proliferation and differentiation. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at post-transcriptional level. Increasing evidences indicate that miRNAs are important regulators in myogenic processes. Here, we reported that the expression of miR-10b-5p steadily decreased during myoblasts proliferation, but significantly increased during myoblasts differentiation. The over-expression of miR-10b-5p promoted myoblasts proliferation and blunted myofiber formation in C2C12 cells, while miR-10b-5p down-regulation showed an opposite result. At the same time, we observed that the down-regulation of nuclear factor of activated T-cells 5 (NFAT5) repressed the differentiation of C2C12 cells, and interestingly, miR-10b-5p could suppress NFAT5 expression. Luciferase activity assays confirmed that miR-10b-5p directly target the 3'-untranslated region (3'-UTR) of NFAT5. Overall, we proposed here a novel insight that miR-10b-5p regulates the proliferation and differentiation of C2C12 myoblasts, and the impact on myogenic differentiation is partly through targeting NFAT5.Abbreviations: NFAT5: nuclear factor of activated T-cells 5; Cyclin B: cycle protein B; Cyclin D1: cycle protein D1; Cyclin E: cycle protein E; CDK4: cyclin-dependent kinase 4; MyoD: myogenic differentiation antigen; MyoG: myogenin; Myf5: myogenic factor 5; MRF4: myogenic regulatory factor 4; MyHC: myosin heavy chain; AQP5: aquaporin-5; CACNA1C: calcium voltage-gated channel subunit alpha1 C; SRF: serum response factor; Pax7: paired box 7; KLF4: Kruppel-like factor 4; 3'-UTR: 3'-untranslated region; GM: growth medium; DM: differentiation medium

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