Cloning of flavonoid 3,5-hydroxylase and 3-hydroxylase homologs from black currant (Ribes nigrum) and their differential expression at various fruit maturation stages
文献类型： 外文期刊
作者： Li, Xing-guo 1 ; Yang, Tian-tian 2 ; Yu, Ze-yuan 1 ; Huo, Jun-wei 1 ; Dong, Qin 1 ; Duan, Ya-dong 3 ; Yang, Guang 3 ;
作者机构： 1.Northeast Agr Univ, Coll Hort & Landscape Architecture, Harbin 150030, Heilongjiang, Peoples R China
2.Northeast Forestry Univ, Coll Wildlife Resources, Harbin 150040, Heilongjiang, Peoples R China
3.Heilongjiang Acad Agr Sci, Berries Res Inst, Suiling 152204, Peoples R China
关键词： Anthocyanin; Flavonoid; Gene cloning; Expression; Blackcurrant
期刊名称：JOURNAL OF FORESTRY RESEARCH （ 影响因子：2.149； 五年影响因子：1.877 ）
ISSN： 1007-662X
年卷期： 2019 年 30 卷 2 期
页码：
收录情况： SCI
摘要： Flavonoid 3-hydroxylase (F3H) and 3,5-hydroxylase (F35H) generate precursor molecules for the synthesis of cyanidin-based anthocyanins (red) and delphinidin-based anthocyanins (blue to purple) in Ribes nigrum L. (black currant). In this study, full-length 1780 and 1650-bp cDNA homologs of RnF35H1 and RnF3H1 from black currant were identified and cloned using a homologous cloning technique. Data revealed that Rn F35H1 and RnF3H1 are homologs that encode enzymes involved in anthocyanin synthesis from different plants, which phylogenetically cluster with the CYP75B and CYP75A families in the P450 superfamily, respectively. The enzymes encoded by these two genes also shared a high homology with flavonoid hydroxylases identified from other plants. Furthermore, RnF35H1 and RnF3H1 levels were upregulated during fruit maturation. RnF35H1 levels were associated with both anthocyanin and soluble carbohydrate levels in blackcurrant, while RnF3H1 expression did not have such an association. The structure and expression patterns of RnF35H1 and RnF3H1 in blackcurrant were also characterized. Further studies should aid understanding of anthocyanin biosynthesis in black currant to develop molecular approaches and manipulate anthocyanin production in blackcurrant.
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