Overexpression of the Eucommia ulmoides Aquaporin, EuPIP1;1, Promotes Leaf Growth, Flowering and Bolting, and Stress Tolerance in Arabidopsis
文献类型： 外文期刊
作者： Chen, Jiajia 1 ; Huang, Yanhui 1 ; Li, Jianrong 1 ; Li, Yan 1 ; Zeng, Xiaofang 1 ; Zhao, Degang 1 ;
作者机构： 1.Guizhou Univ, Coll Life Sci, Key Lab Plant Resource Conservat & Germplasm Inno, Minist Educ,Inst Agrobioengn, Guiyang 550025, Peoples R China
2.Guizhou Acad Agr Sci, Guizhou Plant Conservat Technol Ctr, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China
关键词： Eucommia ulmoides; EuPIP1; 1; leaf development; bolting and flowering; salt resistance; drought resistance
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 19 期
页码：
收录情况： SCI
摘要： Plasma membrane intrinsic protein (PIP) is one of the largest subfamilies of Aquaporins (AQPs) and plays an important role in plant growth and development, and resistance to abiotic stress. In this study, the full length of the EuPIP1;1 cDNA was cloned from Eucommia ulmoides using the rapid amplification of cDNA ends (RACE) method. The EuPIP1;1 gene was induced by drought treatment and expressed in all tested tissues, with the highest expression level in fruit. The subcellular localization showed that EuPIP1;1 was located in the plasma membrane. Constitutive overexpression of EuPIP1;1 in Arabidopsis thaliana could promote leaf growth and development, and accelerate bolting and flowering. Six genes related to growth and flowering (AtPIF4, AtTCP14, AtCRY1, AtCRY2, AtFCA and AtFT) were significantly up-regulated in transgenic lines. Further, EuPIP1;1 gene improved resistance to drought and salt stress in transgenic Arabidopsis. Under drought and salt stress treatment, the transgenic lines had a higher germination rate and accumulation of osmotic substances, lower membrane damage, and could maintain ion homeostasis. Our results suggest that EuPIP1;1 plays an essential role in plant growth and development and in the response to drought and salt stress.
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