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Effects of Bacillus thuringiensis Treatment on Expression of Detoxification Genes in Chlorantraniliprole-Resistant Plutella xylostella

文献类型： 外文期刊

作者： Zolfaghari, Maryam 1 ; Yin, Fei 1 ; Jurat-Fuentes, Juan Luis 3 ; Xiao, Yong 1 ; Peng, Zhengke 1 ; Wang, Jiale 4 ; Yang, Xiangbing 5 ; Li, Zhen-Yu 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

2.Guangdong Acad Agr Sci, Key Lab Green Prevent & Control Fruits & Vegetable, Minist Agr & Rural Affairs, Guangzhou 510640, Peoples R China

3.Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA

4.Guangdong Acad Agr Sci, Inst Qual Stand & Monitoring Technol Agroprod, Guangzhou 510640, Peoples R China

5.USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA

关键词： resistance; Bt-G033; RNAi; gene expression; chlorantraniliprole; diamondback moth

期刊名称：INSECTS （ 影响因子：2.9； 五年影响因子：3.3 ）

ISSN：

年卷期： 2024 年 15 卷 8 期

页码：

收录情况： SCI

摘要： Detoxification genes are crucial to insect resistance against chemical pesticides, yet their expression may be altered by exposure to biopesticides such as spores and insecticidal proteins of Bacillus thuringiensis (Bt). Increased enzymatic levels of selected detoxification genes, including glutathione S-transferase (GST), cytochrome P450 (CYP450), and carboxylesterase (CarE), were detected in chlorantraniliprole (CAP)-resistant strains of the diamondback moth (DBM, Plutella xylostella) from China when compared to a reference susceptible strain. These CAP-resistant DBM strains displayed distinct expression patterns of GST 1, CYP6B7, and CarE-6 after treatment with CAP and a Bt pesticide (Bt-G033). In particular, the gene expression analysis demonstrated significant upregulation of the CYP6B7 gene in response to the CAP treatment, while the same gene was downregulated following the Bt-G033 treatment. Downregulation of CYP6B7 using RNAi resulted in increased susceptibility to CAP in resistant DBM strains, suggesting a role of this gene in the resistant phenotype. However, pretreatment with a sublethal dose of Bt-G033 inducing the downregulation of CYP6B7 did not significantly increase CAP potency against the resistant DBM strains. These results identify the DBM genes involved in the metabolic resistance to CAP and demonstrate how their expression is affected by exposure to Bt-G033.

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