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P450 gene CYP6a13 is responsible for cross-resistance of insecticides in field populations of Spodoptera frugiperda

文献类型： 外文期刊

作者： Chen, Yumei 1 ; Cen, Yongjie 1 ; Liu, Yu 1 ; Peng, Yanan 1 ; Lin, Yiguang 1 ; Feng, Qili 1 ; Xiao, Yong 2 ; Zheng, Sichun 1 ;

作者机构： 1.South China Normal Univ, Guangdong Prov Key Lab Insect Dev Biol & Appl Tech, Guangzhou Key Lab Insect Dev Regulat & Appl Res, Sch Life Sci,Inst Insect Sci & Technol, Guangzhou 510631, Peoples R China

2.Guangdong Acad Agr Sci, Inst Plant Protect, Key Lab Green Prevent & Control Fruits & Vegetable, Minist Agr & Rural Affairs,Guangdong Prov Key Lab, Guangzhou, Peoples R China

关键词： cross-resistance; cytochrome P450; field population; insecticide resistance; Spodoptera frugiperda

期刊名称：INSECT SCIENCE （ 影响因子：4.0； 五年影响因子：3.6 ）

ISSN： 1672-9609

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Continuous and long-term use of traditional and new pesticides can result in cross-resistance among pest populations in different fields. Study on the mechanism of cross-resistance and related genes will help resistance management and field pest control. In this study, the pesticide-resistance mechanism in Spodoptera frugiperda (FAW) was studied with field populations in 3 locations of South China. Field FAW populations were highly resistant to traditional insecticides, chlorpyrifos (organophosphate) and deltamethrin (pyrethroid), and had higher levels of cytochrome P450 activity than a non-resistant laboratory strain. Inhibition of P450 activity by piperonyl butoxide significantly increased the sensitivity of resistant FAW in 3 locations to chlorpyrifos, deltamethrin and chlorantraniliprole (amide), a new type of insecticide, suggesting that P450 detoxification is a critical factor for insecticide resistance in field FAW populations. Transcriptomic analysis indicated that 18 P450 genes were upregulated in the field FAW populations collected in 3 regions and in 2 consecutive years, with CYP6a13, the most significantly upregulated one. Knockdown of CYP6a13 messenger RNA by RNA interference resulted in an increased sensitivity to the 3 tested insecticides in the field FAW. Enzyme activity and molecular docking analyses indicated that CYP6a13 enzyme was able to metabolize the 3 tested insecticides and interact with 8 other types of insecticides, confirming that CYP6a13 is a key cross-resistance gene with a wide range of substrates in the field FAW populations across the different regions and can be used as a biomarker and target for management of FAW insecticide resistance in fields.

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