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The larvae of Phyllotreta striolata share the same olfactory cues for locating Brassicaceae plant with conspecific adults

文献类型： 外文期刊

作者： Xiao, Yong 1 ; Sun, Liang 2 ; Wu, Yuhong 1 ; Wang, Qian 5 ; Zhang, Yongjun 4 ; Jing, Xiangfeng 3 ; Li, Zhenyu 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Key Lab Green Prevent & Control Fruits & Vegetable, Guangdong Prov Key Lab High Technol Plant Protect, Inst Plant Protect,Minist Agr & Rural Affairs, Guangzhou 510640, Peoples R China

2.Chinese Acad Agr Sci, Tea Res Inst, Key Lab Tea Qual & Safety Control, Minist Agr & Rural Affairs, Hangzhou 310008, Peoples R China

3.Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Peoples R China

4.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

5.Zhejiang A&F Univ, Coll Adv Agr Sci, Hangzhou 311300, Peoples R China

关键词： Isothiocyanates; Odorant-binding proteins; Host plant location; Olfaction; Behavioral responses

期刊名称：JOURNAL OF PEST SCIENCE （ 影响因子：4.8； 五年影响因子：5.0 ）

ISSN： 1612-4758

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The sophisticated olfactory system of insects plays crucial roles in host plant location. In comparison with comprehensive studies on the molecular mechanisms of olfactory cues detection in lepidopteran moths, little is known about how coleopteran beetles detect host plant cues. Phyllotreta striolata is a devastating coleopteran pest of Brassicaceae crops, and its larvae feed on roots underground, while its adults destroy leaves aboveground. In this study, we focus on the molecular basis of olfactory cues detection in P. striolata and attempt to determine whether P. striolata larvae share the same specific olfactory cues for host plant location with conspecific adults and whether the detection mechanism is conserved. A two-choice behavioral bioassay was conducted to examine the behavioral responses of P. striolata to different types of isothiocyanates (ITCs), which are the characteristic volatiles of Brassicaceae crops. The results showed that both P. striolata adults and larvae were attracted by allyl ITC, although adults showed a broader behavioral response range. The transcriptome sequencing of P. striolata adults and larvae was performed, and 157 chemosensory genes were identified, among which 6 OBPs, 2 CSPs, 1 OR, 1 IR and 1 GR were found to be preferentially expressed in both P. striolata adults and larvae. Functional studies of PstrOBP9, PstrOBP13 and PstrOBP17, three of the six OBPs that were highly expressed in both adults and larvae, revealed that PstrOBP9 strongly bound allyl ITC and eight other ITCs. Taken together, these findings demonstrated that P. striolata larvae and adults could employ the same olfactory proteins to detect specific plant volatiles for host location, which provides a new perspective on the development of environmentally friendly pest management targeting both P. striolata adults and larvae.

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