The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae
文献类型： 外文期刊
作者： Li, Huan 1 ; Liu, Feng 1 ; Peng, Wei 1 ; Van, Kang 1 ; Zhao, Haixu 1 ; Liu, Ting 1 ; Cheng, Hui 1 ; Chang, Peixi 1 ; Yuan, F 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China
2.Huazhong Agr Univ, Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan, Hubei, Peoples R China
3.Huazhong Agr Univ, Key Lab Dev Vet Diagnost Prod, Minist Agr, Wuhan, Hubei, Peoples R China
4.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan, Hubei, Peoples R China
关键词： Actinobacillus pleuropneumoniae; CpxA/CpxR; biofilm; rpoE; virulence
期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：5.293； 五年影响因子：5.882 ）
ISSN： 2235-2988
年卷期： 2018 年 8 卷
页码：
收录情况： SCI
摘要： Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and the regulator CpxR, is known to be involved in the biofilm formation and virulence of Escherichia coli. However, the role of CpxA/CpxR remained unclear in Actinobacillus pleuropneumoniae, a bacterial pathogen that can cause porcine contagious pleuropneumonia (PCP). In this report, we show that CpxA/CpxR contributes to the biofilm formation ability of A. pleuropneumoniae. Furthermore, we demonstrate that CpxA/CpxR plays an important role in the expression of several biofilm-related genes in A. pleuropneumoniae, such as rpoE and pgaC. Furthermore, The results of electrophoretic mobility shift assays (EMSAs) and DNase I footprinting analysis demonstrate that CpxR-P can regulate the expression of the pgaABCD operon through rpoE. In an experimental infection of mice, the animals infected with a cpxA/cpxR mutant exhibited delayed mortality and lower bacterial loads in the lung than those infected with the wildtype bacteria. In conclusion, these results indicate that the CpxA/CpxR TCS plays a contributing role in the biofilm formation and virulence of A. pleuropneumoniae.
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