您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and Functional Characterization of R3 MYB Transcription Factor Genes in Soybean

文献类型： 外文期刊

作者： Xun, Hongwei 1 ; Zhang, Zhibing 1 ; Zhou, Yunxiao 1 ; Qian, Xueyan 3 ; Dong, Yingshan 3 ; Feng, Xianzhong 4 ; Pang, Ji 1 ;

作者机构： 1.Northeast Normal Univ, Key Lab Mol Epigenet, MOE, Changchun 130024, Jilin, Peoples R China

2.Northeast Normal Univ, Inst Cytol & Genet, Changchun 130024, Jilin, Peoples R China

3.Jilin Acad Agr Sci, Changchun 130033, Jilin, Peoples R China

4.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Changchun 130100, Jilin, Peoples R China

关键词： Arabidopsis; GmTCL1; R3 MYBs; Soybean; TCL1; Transcription factor; Trichome

期刊名称：JOURNAL OF PLANT BIOLOGY （ 影响因子：2.434； 五年影响因子：2.455 ）

ISSN： 1226-9239

年卷期： 2018 年 61 卷 2 期

页码：

收录情况： SCI

摘要： In Arabidopsis, trichome formation is regulated by a MYB-bHLH-WD40 (MBW) transcriptional activator complex, which can activate the expression of GLABRA2 (GL2) and R3 MYB genes. GL2 is required for trichome formation, whereas R3 MYBs inhibit trichome formation by blocking the formation of the MBW complex, thus inhibiting the expression of GL2. By using the amino acid sequence of the Arabidopsis R3 MYB transcription factor TRICHOMELESS1 (TCL1) to BLAST the soybean (Glycine max) protein database, we found that there are a total of six R3 MYB genes in soybean, namely Glycine max TRICHOMELESS1 through 6 (GmTCL1-GmTCL6). By generating transgenic plants, we found that trichome formation in soybean plants overexpressing each of the GmTCLs remained largely unchanged, and the expression of putative GL1 and GL2 genes in the transgenic plants was unaffected. However, all the GmTCLs interacted with GLABRA3 (GL3) in transfected Arabidopsis protoplasts, expression each of the GmTCLs in Arabidopsis inhibited trichome formation, and the expression levels of GL1 and GL2 were greatly reduced in the Arabidopsis transgenic plants. Moreover, phenotypic complementary analysis showed that GmTCL1 is functionally equivalent to TCL1. Taken together, these results suggest that GmTCLs are functional R3 MYBs, however, they do not regulate trichome formation in soybean.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台