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Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome

文献类型： 外文期刊

作者： Xin, Miaomiao 1 ; Shaliutina-Kolesova, Anna 1 ; Sterba, Jan 3 ; Konik, Peter 3 ; Boryshpolets, Sergii 1 ; Rodina, M 1 ;

作者机构： 1.Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Res Inst Fish Culture & Hydrobiol, Zatisi 728-2, Vodnany 38925, Czech Republic

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Sino Czech Joint Lab Fish Conservat & Biotechnol, Wuhan, Hubei, Peoples R China

3.Univ South Bohemia Ceske Budejovice, Fac Sci, Inst Chem & Biochem, Branisovska 1760, Ceske Budejovice 37005, Czech Republic

4.Acad Sci Czech Republ, Inst Parasitol, Biol Ctr, Branisovska 31, CR-37005 Ceske Budejovice, Czech Republic

5.Chinese Acad Sci, CAS Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Peoples R China

关键词： Sperm quality; Cryodamage; Sperm proteins

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

ISSN： 0378-4320

年卷期： 2018 年 192 卷

页码：

收录情况： SCI

摘要： Fish sperm cryopreservation is a well-established technique allowing for artificial insemination on a commercial scale. The extent of proteome alterations in seminal plasma and sperm due to cryopreservation, however, is not known. This study was conducted to evaluate the effect of cryopreservation on motility variables of sterlet Acipenser ruthenus sperm and to detect the differences in protein profiles of fresh and cryopreserved sterlet sperm and seminal plasma. Fresh sperm had 89 +/- 3% motility and 160 +/- 14 mu m/s curvilinear velocity at 15 s post-activation. The motility rate of cryopreserved sperm (37 +/- 5%) was less at 15 s post-activation. No difference (ANOVA; P > 0.05) in mean curvilinear velocity of fresh and cryopreserved sperm was detected. The protein profiles of seminal plasma and sperm were characterized using comparative proteomics to determine the influence of cryopreservation. Six altered protein spots in seminal plasma and thirteen altered spots in sperm were detected in fresh and thawed sperm. Subsequent protein characterization suggested that the proteins identified were involved in sperm metabolism, cytoskeleton, and stress response. The results broaden the understanding of the effects of cryopreservation and identify the proteins associated with cryo-injury. These data may help to determine the function of altered proteins and provide new insights into improving sperm cryopreservation.

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