Targeted inactivation of antibiotic-resistant Escherichia coli and Pseudomonas aeruginosa in a soil-lettuce system by combined polyvalent bacteriophage and biochar treatment
文献类型： 外文期刊
作者： Ye, Mao 1 ; Sun, Mingming 2 ; Zhao, Yuanchao 2 ; Jiao, Wentao 1 ; Xia, Bing 4 ; Liu, Manqiang 2 ; Feng, Yanfang 4 ; Zhang 1 ;
作者机构： 1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Soil Ecol Lab, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China
3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China
4.Anhui Acad Environm Sci Res, Hefei 230022, Anhui, Peoples R China
5.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjing 210014, Jiangsu, Peoples R China
6.Minist Environm Protect China, Nanjing Inst Environm Sci, Nanjing 210042, Jiangsu, Peoples R China
关键词： Polyvalent bacteriophage therapy; Biochar; Antibiotic resistance genes; Escherichia coli K-12; Pseudomonas aeruginosa PAO1
期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：8.071； 五年影响因子：8.35 ）
ISSN： 0269-7491
年卷期： 2018 年 241 卷
页码：
收录情况： SCI
摘要： High abundances of antibiotic-resistant pathogenic bacteria (ARPB) and antibiotic resistance genes (ARGs) in agricultural soil-plant systems have become serious threats to human health and environmental safety. Therefore, it is crucial to develop targeted technology to control existing antibiotic resistance (AR) contamination and potential dissemination in soil-plant systems. In this work, polyvalent bacteriophage (phage) therapy and biochar amendment were applied separately and in combination to stimulate ARPB/ARG dissipation in a soil-lettuce system. With combined application of biochar and polyvalent phage, the abundance of Escherichia coli K-12 (tet(R)) and Pseudomonas aeruginosa PAO1 (amp(R) + fos(R)) and their corresponding ARGs (tetM, tetQ, tetW, ampC, and fosA) significantly decreased in the soil after 63 days' incubation (p < 0.05). Similar results for endophytic K-12 and PAO1, and ARGs, were also obtained in lettuce tissues following combined treatment. Additionally, high throughput sequencing revealed that biochar and polyvalent phage synergetically improved the structural diversity and functional stability of the indigenous bacterial communities in soil and the endophytic ones in lettuce. Hence, this work proposes a novel biotechnology that combines biochar amendment and polyvalent phage therapy to achieve targeted inactivation of ARPB, which stimulates ARG dissipation in soil-lettuce systems. (C) 2018 Elsevier Ltd. All rights reserved.
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