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Comparative RNA-Seq analysis reveals a critical role for brassinosteroids in rose (Rosa hybrida) petal defense against Botrytis cinerea infection

文献类型： 外文期刊

作者： Liu, Xintong 1 ; Cao, Xiaoqian 1 ; Shi, Shaochuan 1 ; Zhao, Na 1 ; Li, Dandan 1 ; Fang, Peihong 1 ; Chen, Xi 2 ; Qi, Weic 1 ;

作者机构： 1.China Agr Univ, Coll Hort, Dept Ornamental Hort, Beijing Key Lab Dev & Qual Control Ornamental Cro, Yuanmingyuan Xilu 2, Beijing 100193, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Biotechnol, Prov Key Lab Agrobiol, Zhonglingjie 50, Nanjing 210014, Jiangsu, Peoples R China

关键词： Rose; Botrytis cinerea; Transcriptome; Cell surface receptors; Transcription factors; Brassinosteroid; Immune response

期刊名称：BMC GENETICS （ 影响因子：2.797； 五年影响因子：3.263 ）

ISSN： 1471-2156

年卷期： 2018 年 19 卷

页码：

收录情况： SCI

摘要： Background: One of the most popular ornamental plants worldwide, roses (Rosa sp.), are very susceptible to Botrytis gray mold disease. The necrotrophic infection of rose petals by B. cinerea causes the collapse and death of these tissues in both the growth and post-harvest stages, resulting in serious economic losses. To understand the molecular basis of rose resistance against B. cinerea, we profiled the petal transcriptome using RNA-Seq technology. Results: We identified differentially transcribed genes (DTGs) in petals during B. cinerea infection at 30 h post inoculation (hpi) and/or 48 hpi. Gene ontology term enrichment and pathway analyses revealed that metabolic, secondary metabolite biosynthesis, plant-pathogen interaction, and plant hormone signal transduction pathways were involved. The expression of 370 cell-surface immune receptors was upregulated during infection. In addition, 188 genes encoding transcription factors were upregulated, particularly in the ERF, WRKY, bHLH, MYB, and NAC families, implying their involvement in resistance against B. cinerea. We further identified 325 upregulated DTGs in the hormone signal transduction pathways. Among them, the brassinosteroid (BR)-related genes were the most significantly enriched. To confirm the role of BR in Botrytis resistance, exogenous BR was applied to rose flowers before the inoculation of B. cinerea, which enhanced the defense response in these petals. Conclusions: Our global transcriptome profiling provides insights into the complex gene regulatory networks mediating the rose petal response to B. cinerea. We further demonstrated the role of the phytohormone BR in the resistance of petals to necrotrophic fungal pathogens.

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