文献类型： 外文期刊

作者： Liu, Rong ¹ ; Hu, Chaoqin ² ; Gao, Dan ³ ; Li, Mengwei ¹ ; Yuan, Xingxing ⁴ ; Chen, Liyang ³ ; Shu, Qin ¹ ; Wang, Zonghe ¹ ; Yang, Xin ² ; Dai, Zhengming ² ; Yu, Haitian ² ; Yang, Feng ² ; Zheng, Aiqing ² ; Lv, Meiyuan ² ; Garg, Vanika ⁵ ; Jiao, Chengzhi ³ ; Zhang, Hongyan ⁶ ; Hou, Wanwei ⁶ ; Teng, Changcai ⁶ ; Zhou, Xianli ⁶ ; Du, Chengzhang ⁸ ; Xiang, Chao ⁹ ; Xu, Dongxu ¹⁰ ; Tang, Yongsheng ¹¹ ; Chitikineni, Annapurna ⁵ ; Duan, Yinmei ¹² ; Maalouf, Fouad ¹³ ; Agrawal, Shiv Kumar ¹³ ; Wei, Libin ¹⁴ ; Zhao, Na ¹⁴ ; Barmukh, Rutwik ⁵ ; Li, Xiang ¹⁵ ; Wang, Dong ¹⁶ ; Ding, Hanfeng ¹⁶ ; Liu, Yujiao ⁶ ; Chen, Xin ⁴ ; Varshney, Rajeev K. ⁵ ; He, Yuhua ² ; Zong, Xuxiao ¹ ; Yang, Tao ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, State Key Lab Crop Gene Resources & Breeding, Beijing 100081, Peoples R China

2.Yunnan Acad Agr Sci, Food Crops Res Inst, Kunming 650205, Yunnan, Peoples R China

3.Smartgenom Technol Inst, Tianjin 301700, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Jiangsu, Peoples R China

5.Murdoch Univ, Food Futures Inst, State Agr Biotechnol Ctr, Ctr Crop & Food Innovat, Murdoch, WA 6150, Australia

6.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Qinghai, Peoples R China

7.Qinghai Univ, Qinghai Acad Agr & Forestry Sci, Xining 810016, Qinghai, Peoples R China

8.Chongqing Acad Agr Sci, Chongqing 401329, Peoples R China

9.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China

10.Zhangjiakou Acad Agr Sci, Zhangjiakou 075032, Hebei, Peoples R China

11.Qujing Acad Agr Sci, Qujing 655000, Yunnan, Peoples R China

12.Dali Acad Agr Sci, Dali 671005, Yunnan, Peoples R China

13.ICARDA, Beirut 11082010, Lebanon

14.Jiangsu Yanjiang Inst Agr Sci, Nantong 226541, Jiangsu, Peoples R China

15.Yunnan Acad Tobacco Agr Sci, Yuxi 653100, Yunnan, Peoples R China

16.Shandong Acad Agr Sci, Inst Crop Germplasm Resources, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Shandong, Peoples R China

关键词： Faba bean; Short-wing petal; Genome assembly; Genomic diversity; GWAS; Yield-related loci; Breeding; Improvement

期刊名称：GENOME BIOLOGY （ 影响因子：9.4； 五年影响因子：16.3 ）

ISSN： 1474-760X

年卷期： 2025 年 26 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundA comprehensive study of the genome and genetics of superior germplasms is fundamental for crop improvement. As a widely adapted protein crop with high yield potential, the improvement in breeding and development of the seeds industry of faba bean have been greatly hindered by its giant genome size and high outcrossing rate.ResultsTo fully explore the genomic diversity and genetic basis of important agronomic traits, we first generate a de novo genome assembly and perform annotation of a special short-wing petal faba bean germplasm (VF8137) exhibiting a low outcrossing rate. Comparative genome and pan-genome analyses reveal the genome evolution characteristics and unique pan-genes among the three different faba bean genomes. In addition, the genome diversity of 558 accessions of faba bean germplasm reveals three distinct genetic groups and remarkable genetic differences between the southern and northern germplasms. Genome-wide association analysis identifies several candidate genes associated with adaptation- and yield-related traits. We also identify one candidate gene related to short-wing petals by combining quantitative trait locus mapping and bulked segregant analysis. We further elucidate its function through multiple lines of evidence from functional annotation, sequence variation, expression differences, and protein structure variation.ConclusionsOur study provides new insights into the genome evolution of Leguminosae and the genomic diversity of faba bean. It offers valuable genomic and genetic resources for breeding and improvement of faba bean.