文献类型： 外文期刊

作者： Zhou, Zhixiong ¹ ; Chen, Lin ² ; Dong, Chuanju ³ ; Peng, Wenzhu ² ; Kong, Shengnan ² ; Sun, Jinsheng ¹ ; Pu, Fei ² ; Chen ¹ ;

作者机构： 1.Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China

2.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen 361102, Peoples R China

3.Henan Normal Univ, Coll Fishery, Xinxiang 453007, Henan, Peoples R China

4.Chinese Acad Fishery Sci, CAFS Key Lab Aquat Genom, Ctr Appl Aquat Genom, Beijing 100141, Peoples R China

5.Chinese Acad Fishery Sci, Beijing Key Lab Fishery Biotechnol, Ctr Appl Aquat Genom, Beijing 100141, Peoples R China

6.Henan Acad Fishery Sci, Zhengzhou 450044, Henan, Peoples R China

7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China

关键词： Yellow River carp; GWAS; Scattered scale; fgfr1a1; Common carp

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.619； 五年影响因子：3.322 ）

ISSN： 1436-2228

年卷期： 2018 年 20 卷 5 期

页码：

收录情况： SCI

摘要： Common carp (Cyprinus carpio) is one of the most widely studied fish species due to its great economic value and strong environmental adaptability. Scattered scale, a typical phenotype of the mirror carp that is derived from Europe, has never been observed in the Yellow River carp previously. We recently identified approximately one fourth of the F1 progenies displaying scattered scale in a full-sib Yellow River carp family in our breeding program, despite both parents that showed wild type with normal scale patterns. This family provides us unique materials to investigate the genetic basis underlying the abnormal scale mutant in Yellow River carp population. Genome-wide association study (GWAS) and association mapping were performed based on genome-wide single nucleotide polymorphisms (SNP) genotyped with common carp 250K SNP genotyping array in 82 samples of the Yellow River carp family. We identified a 1.4Mb genome region that was significantly associated with abnormal scattered scale patterns. We further identified a deletion mutation in fibroblast growth factor receptor 1 a1 (fgfr1a1) gene within this genome region. Amplification and sequencing analysis of this gene revealed a 311-bp deletion in intron 10 and exon 11, which proved that fgfr1a1 could be the causal gene responsible for abnormal scattered scale in the Yellow River carp family. Since similar fragment mutation with 306-bp and 310-bp deletions had been previously reported as causal mutation of scattered scale patterns in the mirror carp, we speculate that either the deletion mutation was introduced from Europe-derived mirror carp or the deletion independently occurred in the mutation hotspot in fgfr1a1 gene. The results provided insights into the genetic basis of scale pattern mutant in Yellow River carp population, which would help us to eliminate the recessive allele of the abnormal scale patterns in Yellow River carp population by molecular marker-assisted breeding.