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Dnd is required for primordial germ cell specification in Oryzias celebensis

文献类型： 外文期刊

作者： Zhu, Tianyu 1 ; Gui, Lang 1 ; Zhu, Yefei 1 ; Li, Yu 4 ; Li, Mingyou 1 ;

作者机构： 1.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China

2.Shanghai Ocean Univ, Int Res Ctr Marine Biosci, Minist Sci & Technol, Shanghai 201306, Peoples R China

3.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

4.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab Ocean & Polar Fisheries, Minist Agr, Shanghai 200090, Peoples R China

关键词： Dnd; Oryzias celebensis; PGCs; Specification

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2018 年 679 卷

页码：

收录情况： SCI

摘要： Dead end (dnd) is a germ plasm component that plays an essential role for primordial germ cell (PGC) development in vertebrates. Previously, we have found that dnd is the first fish PGC specifier in medaka. Here, we present an additional evidence that dnd is the determinant for PGC specification in Oryzias celebensis. In adult tissues, the O. celebensis dnd (Ocdnd) RNA shows germ cells specific expression in gonads. In the testis, Ocdnd RNA is strongly detected in spermatogonia and meiotic cells and gradually decreases during the spermatogenesis. In the ovary, Ocdnd RNA is present throughout oogenesis. In the embryos, Ocdnd RNA is maternally provided and asymmetrically localized to prominent particles of presumptive PGCs before gastrulation stage and restricted to PGCs subsequently. In addition, Ocdnd 3' UTR can induce specific and stabilized GFP reporter expression in PGCs. Furthermore, knockdown of Ocdnd by morpholino (MO) injection abolishes the PGCs formation and this can be rescued by co-injection of medaka dnd (Oldnd) mRNA. More importantly, overexpression of Oldnd mRNA surprisingly boosts PGCs number. These results provide insights into function of dnd as a conserved specifier of PGCs in the genus Oryzias.

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