文献类型: 外文期刊
作者: Ding, Yuan 1 ; Hua, Xiude 1 ; Du, Mei 1 ; Yang, Qian 1 ; Hou, Lina 1 ; Wang, Limin 1 ; Liu, Fengquan 1 ; Gonzalez-Sapienz 1 ;
作者机构: 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
2.State & Local Joint Engn Res Ctr Green Pesticide, Nanjing 210095, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China
4.Univ Republica, Fac Quim, Inst Higiene, Catedra Inmunol, Montevideo 11600, Uruguay
期刊名称:ANALYTICAL CHEMISTRY ( 影响因子:6.986; 五年影响因子:6.755 )
ISSN: 0003-2700
年卷期: 2018 年 90 卷 23 期
页码:
收录情况: SCI
摘要: Peptidomimetic and anti-immunocomplex peptides, which can be readily isolated from a phage-display library, have shown great potential for small-molecule immunoassay development because they typically improve the sensitivity and avoid the use of chemical haptens as coatings or tracer antigens. However, phage-borne peptides are unconventional immunoassay reagents, which greatly limits their use in commercial applications, and require secondary reagents for detection. In order to overcome these limitations, we used C2-15, a peptidomimetic of imidaclothiz, as a model peptide fused to emerald-green fluorescent protein (EmGFP) at the N-terminus (C2-15-EmGFP) and C-terminus (EmGFP-C2-15) to generate novel fluorescent-peptide tracers. Both recombinant fluorophores reacted with similar affinity to the anti-imidaclothiz monoclonal antibody 1E(7), but because of its higher expression, C2-15-EmGFP was chosen to develop a competitive Fluorescent peptide tracer magnetic-separation fluorescence immunoassay (MSFIA). After a competitive step with the analyte, the C2-15-EmGFP-antibody complex bound to the magnetic beads was separated with a magnet, and because of the fast dissociation of the peptide-antibody interaction, the fluorescence signal was detected following the spontaneous dissociation of the complex in fresh buffer. The concentration of imidaclothiz causing the 50% inhibitory concentration (IC50) was 11.00 ng mL(-1), and the MSFIA performed with excellent recovery and had a good correlation with high-performance liquid chromatography in different matrices.
- 相关文献
作者其他论文 更多>>
-
First Report of Bacterial Fruit Blotch Caused by Kosakonia cowanii on Trichosanthis fructus in Jiangsu Province, China
作者:Chen, Xian;Tang, Bao;Zhao, Yancun;Liu, Fengquan;Chen, Xian;Tang, Bao;Laborda, Pedro
关键词:bacterial fruit blotch; Kosakonia cowanii; Trichosanthis fructus
-
Resistance patterns and molecular basis to ACCase-inhibiting herbicides
作者:Yang, Qian;Wei, Tian;Zhu, Jinlei;Lv, Min;Deng, Wei;Liu, Longwei;Yang, Xia;Li, Yongfeng
关键词:ACCase gene mutation; cross-resistance; metamifop; target site-based resistance
-
Unveiling the Role of Diffusible Signal Factor-Family Quorum Sensing Signals in Regulating Behavior of Xanthomonas and Lysobacter
作者:Li, Kaihuai;Ma, Chaoyun;Xiong, Chunlan;Zhou, Xue;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Ma, Chaoyun;Xiong, Chunlan;Zhou, Xue;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Wang, Yong;Mao, Yahui;Liu, Fengquan
关键词:diffusible signal factor; Lysobacter enzymogenes; quorum sensing; Xanthomonas campestris
-
S-nitrosylation of a receptor-like cytoplasmic kinase regulates plant immunity
作者:Cui, Beimi;Liu, Fengquan;Cui, Beimi;Liu, Fengquan;Cui, Beimi;Pan, Qiaona;Loake, Gary J.;Cui, Wenqiang;Wang, Yiqin;Cui, Wenqiang;Yuan, Shuguang;Wang, Yiqin;Loake, Verity I. P.;Loake, Gary J.
关键词:
-
Oomycete Nudix effectors display WY-Nudix conformation and mRNA decapping activity
作者:Guo, Baodian;Wang, Bangwei;Wang, Haonan;Kong, Guanghui;Dong, Suomeng;Wang, Yuanchao;Guo, Baodian;Wang, Bangwei;Liu, Fengquan;Hu, Qinli;Yao, Deqiang;Han, Chenyang;Liu, Fengquan;Xing, Weiman
关键词:
-
Evaluating the Efficacy of Hymexazol in Controlling Globisporangium spinosum, the Newly Identified Pathogen Causing Root Rot in Houttuynia cordata
作者:Zhou, Xue;Xiong, Chunlan;Ma, Chaoyun;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Zhou, Xue;Xiong, Chunlan;Ma, Chaoyun;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Tu, Yupei;Wang, Yong;Li, Kaihuai;Liu, Fengquan
关键词:Houttuynia cordata; hymexazol; oomycete; root rot
-
Removal of aqueous Cr(VI) by green synthesized sulfide iron nanoparticles loaded corn straw biochar: Performance, mechanism, and DFT calculations
作者:Rong, Kun;Li, Xueping;Yang, Qian;Zhang, Zaiwang;Li, Ruimin;Zhao, Lingzi;Zheng, Huanqiang;Rong, Kun;Liu, Zhaohui;Yao, Qiang
关键词:Sulfide modification; Green synthesis; Biochar; DFT; Aqueous Cr(VI)