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Screening of Streptococcus Suis serotype 2 resistance genes with GWAS and transcriptomic microarray analysis

文献类型： 外文期刊

作者： Ma, Zhe 1 ; Zhu, Haodan 4 ; Su, Yiqi 1 ; Meng, Yu 1 ; Lin, Huixing 1 ; He, Kongwang 4 ; Fan, Hongjie 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Jiangsu, Peoples R China

2.Minist Agr Key Lab Anim Bacteriol, Nanjing 210095, Jiangsu, Peoples R China

3.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

4.Jiangsu Acad Agr Sci, Nanjing 210095, Jiangsu, Peoples R China

关键词： Streptococcus suis serotype 2; Resistance genes; Genome-wide association study; Transcriptome analysis

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2018 年 19 卷

页码：

收录情况： SCI

摘要： BackgroundSwine streptococcosis has caused great economic loss in the swine industry, and the major pathogen responsible for this disease is Streptococcus Suis serotype 2 (SS2). Disease resistance breeding is a fundamental way of resolving this problem. With the development of GWAS and transcriptomic microarray technology, we now have powerful research tools to identify SS2 resistance genes.ResultsIn this research, we generated an F-2 generation of SS2 resistant C57BL/6 and SS2 susceptive A/J mice. With the F-2 generation of these two mice strains and GWAS analysis, we identified 286 significant mouse genome SNPs sites associated with the SS2 resistance trait. Gene expression profiles for C57BL/6 and A/J were analyzed under SS2 infection pressure by microarray. In total, 251 differentially expressed genes were identified between these two mouse strains during SS2 infection. After combining the GWAS and gene expression profile data, we located two genes that were significantly associated with SS2 resistance, which were the UBA domain containing 1 gene (Ubac1) and Epsin 1 gene (Epn 1). GO classification and over-representation analysis revealed nine up-regulated related to immune function, which could potentially be involved in the C57BL/6 SS2 resistance trait.ConclusionThis is the first study to use both SNP chip and gene express profile chip for SS2 resistance gene identification in mouse, and these results will contribute to swine SS2 resistance breeding.

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