A surface protein identified from Streptococcus suis serotype 2 exhibits neutrophil-resistant ability via its polysaccharide capsule
文献类型： 外文期刊
作者： Ma, Fang 1 ; Wang, Guangyu 3 ; Ma, Zhe 1 ; Lin, Huixing 1 ; Fan, Hongjie 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Natl Res Ctr Engn & Technol Vet Biol, Nanjing 210014, Peoples R China
3.Nanjing Univ Finance & Econ, Coll Food Sci & Engn, Nanjing 210023, Peoples R China
4.Anhui Sci & Technol Univ, Coll Anim Sci, Fengyang 233100, Peoples R China
关键词： Streptococcus suis serotype 2; Host-pathogen interactions; Virulence factor; Neutrophil-resistant; Polysaccharide capsule
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）
ISSN： 0882-4010
年卷期： 2025 年 199 卷
页码：
收录情况： SCI
摘要： Streptococcus suis serotype 2 (SS2) is an emerging zoonotic agent responsible for a variety of diseases. The septicemia caused by SS2 suggests that it can evade the bactericidal effects of innate immune cells. However, the mechanisms by which SS2 evades innate immunity remain largely unknown. Neutrophils are critical components of innate immunity for antimicrobial defense. In this study, we identified a cell surface protein (CSP) insertion mutant in an SS2 transposon mutant library that significantly induces neutrophil extracellular traps (NETs) and shows poor survival compared to the parent SS2 in the bloodstream. CSP deletion mutant (Delta CSP) was constructed and it exhibits a defective polysaccharide capsule and enhanced biofilm formation. Although Delta CSP induces increased NET release and ROS formation, its survival and proliferation within neutrophils and NETs are significantly reduced. Additionally, Delta CSP stimulates extracellular signal-regulated kinase 1/2 (ERK1/2) signaling in neutrophils, which may enhance neutrophil bactericidal activity. Importantly, purified polysaccharide capsule from SS2 can inhibit neutrophil bactericidal effects. This study identifies bacterial virulence factors that prevent SS2 clearance by neutrophils, offering potential antigens or drug targets for the prevention and control of swine streptococcosis.
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