Nano-adjuvant based on lipo-imiquimod self-assembly for enhanced foot-and-mouth disease virus vaccine immune responses via intradermal immunization
文献类型： 外文期刊
作者： Yin, Wenzhu 1 ; Xu, Zeyu 2 ; Ma, Fang 1 ; Deng, Bihua 1 ; Zhao, Yanhong 1 ; Zuo, Xiaoxin 1 ; Wang, Haiyan 1 ; Lu, Yu 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing 210014, Peoples R China
2.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
3.GuoTai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 225300, Peoples R China
关键词： Imiquimod; Lipidation; Self-assembly; Nano-adjuvant; Intradermal immunity; Foot-and-mouth vaccine
期刊名称：MATERIALS TODAY BIO （ 影响因子：10.2； 五年影响因子：10.2 ）
ISSN： 2590-0064
年卷期： 2025 年 31 卷
页码：
收录情况： SCI
摘要： Excellent adjuvants and proper immunization routes play pivotal roles in activating a robust immune response. Nano-adjuvants have the advantages of enhancing immunogenicity, targeting delivery, and improving stability to provide a new solution for vaccine delivery. In this work, we designed and synthesized a pro-immunostimulant of liposolubility imiquimod derivative IMQP, which was synthesized by reaction of palmitoyl chloride with parent imiquimod (IMQ). Using an inactivated foot-and-mouth disease virus (FMDV) as antigen, and the as- synthesized IMQP containing long carbon chain as nano-adjuvant, we formulated a self-assembled foot-andmouth disease nano-vaccine (IMQP@FMDV) by re-precipitation method for intradermal (I.D.) immunity vaccination. Because of its small size (similar to 131.75 +/- 41.70 nm) and fat-soluble, the as-fabricated lipid nanoparticles (LNPs) showed promising potential for efficient delivery of antigens to immune cells. Also, lysosomal escape was confirmed by co-localization dendritic cells (DCs). Our findings demonstrated that IMQP nano-adjuvant greatly promoted the expression and secretion of cytokines and chemokines with a balance Th1/Th2 immune response via the I.D. administration. Meanwhile, due to the slowly releasing of IMQ by the hydrolysis of IMQP, IMQP persistently stimulated antigen-presenting cells (APCs) maturation and promoted antigen presentation, and subsequently induced the activation of the related downstream NF-kappa B and MAPK pathways of the TLR7 signaling pathway, thereby stimulated a robust both humoral and cellular immune response.
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