您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of Carassius auratus gibelio liver cell proteins interacting with the GABA(A) receptor 2 subunit using a yeast two-hybrid system

文献类型： 外文期刊

作者： Ma, Rong-Rong 1 ; Sun, Jing 1 ; Fang, Wen-Hong 4 ; Dong, Ya-Ping 1 ; Ruan, Ji-Ming 5 ; Yang, Xian-Le 1 ; Hu, Kun 1 ;

作者机构： 1.Shanghai Ocean Univ, Natl Pathogen Collect Ctr Aquat Anim, Shanghai, Peoples R China

2.Shanghai Ocean Univ, Shanghai Collaborat Innovat Aquat Anim Genet & Br, Shanghai, Peoples R China

3.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai, Peoples R China

4.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab Fisheries Ecol Yangtze Estuary, Shanghai, Peoples R China

5.Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang, Jiangxi, Peoples R China

6.Shanghai Ocean Univ, Coll Fisheries & Life Sci, 999 Hucheng Huan Rd, Shanghai 201306, Peoples R China

关键词： Carassius auratus gibelio; GABA(A) receptor 2 subunit; Liver cDNA library; Yeast two-hybrid; Protein-protein interaction

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2019 年 45 卷 1 期

页码：

收录情况： SCI

摘要： The -aminobutyric acid type A (GABA(A)) receptor is an important pentameric inhibitory neurotransmitter receptor, and the 2 subunit of this receptor plays a key role in potentiation of the GABA(A) response. We previously detected that the expression of GABA(A) receptor in the livers of Carassius auratus gibelio significantly increased after medication (avermectin and difloxacin treatment). In order to better understand the mechanism of action of the GABA(A) receptor 2 subunit in the livers of C. auratus gibelio, we constructed a C. auratus gibelio liver cDNA library (the titer value of 1.2x10(6)cfu/mL) and identified the proteins that interact with the GABA(A) receptor 2 subunit by using a yeast two-hybrid assay. The yeast two-hybrid screening yielded seven positive clones, namely, prelid3b, cdc42, sgk1, spg21, proteasome, chia.5, and AP-3 complex subunit beta-1, all of which have been annotated by the NCBI database. The functions of these proteins are complex; therefore, additional studies are required to determine the specific interactions of these proteins with the GABA(A) receptor 2 subunit in the liver of C. auratus gibelio. Although the interactions identified by the yeast two-hybrid system should be considered as preliminary results, the findings of this study may provide further direction and a foundation for future research focusing on the mechanisms of the GABA(A) receptor 2 subunit in C. auratus gibelio livers.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台