您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Antibacterial activity and mechanism of ThDP analogs against rice brown stripe pathogen Acidovorax avenae subsp. avenae RS-1

文献类型： 外文期刊

作者： Wang, Xiao Xuan 1 ; Qi, Hang Ying 2 ; Chen, Jie 1 ; Yang, Ying Zi 1 ; Qiu, Wen 1 ; Wang, Wei 3 ; Zou, Peng 3 ; Li, Bin 1 ; W 1 ;

作者机构： 1.Zhejiang Univ, Inst Biotechnol, Key Lab Mol Biol Crop Pathogens & Insects, State Key Lab Rice Biol,Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China

2.Zhuji Extens & Serv Ctr Agr Tech, Zhuji 310020, Peoples R China

3.Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol CCNU, Wuhan 430079, Hubei, Peoples R China

4.Cent China Normal Univ, Coll Chem, Wuhan 430079, Hubei, Peoples R China

5.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Chemical; Rice bacterial brown stripe pathogen; Gene expression; Action mode

期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.729； 五年影响因子：1.681 ）

ISSN： 1125-4653

年卷期： 2019 年 101 卷 1 期

页码：

收录情况： SCI

摘要： Thiamin diphosphate (ThDP) analogs have been designed and synthesized based on the ThDP binding site of the pyruvate dehydrogenase multienzyme complex E1 of Escherichia coli. This study investigated the effect of 66 novel ThDP analogs on rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae strain RS-1. Results indicated that three of the 66 ThDP analogs (designated as 20, 21 and 53) significantly inhibited the in vitro growth of strain RS-1. However, no obvious cell lysis and destruction was found for this rice pathogenic bacterium, which were supported by morphological evidence of transmission electron microscope. In contrast, the three ThDP analogs significantly reduced biofilm formation and the activity of the pyruvate dehydrogenase of strain RS-1. Furthermore, the differential expression of ThDP target gene and 20 secretion system related genes were revealed by using quantitative real-time PCR. Among these, the expressions of ThDP target gene and VgrG-5 under the treatment of ThDP 20 were strongly induced compared to the control, which indicated that the antibacterial mechanism of ThDP analogs may be mainly due to the changed expression of ThDP target gene and secretion system related genes rather than causing damage to cell membrane. Taken together, the application of synthesized ThDP analogs might be a tractable strategy to overcome the pathogen of rice bacterial brown stripe.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台