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Banana bunchy top virus (BBTV) nuclear shuttle protein interacts and re-distributes BBTV coat protein in Nicotiana benthamiana

文献类型： 外文期刊

作者： Ji, Xiao-long 1 ; Yu, Nai-tong 1 ; Qu, Ling 1 ; Li, Bin-bin 1 ; Liu, Zhi-xin 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Minist Agr & Rural Affairs,Hainan Prov Key Lab Mi, Haikou 571101, Hainan, Peoples R China

关键词： Banana bunchy top virus; CP; NSP; Intracellular localization; Interaction; Co-IP

期刊名称：3 BIOTECH （ 影响因子：2.406； 五年影响因子：3.203 ）

ISSN： 2190-572X

年卷期： 2019 年 9 卷 4 期

页码：

收录情况： SCI

摘要： Banana bunchy top virus (BBTV) is a circular single-stranded DNA virus with multi-components. The knowledge about interaction between viral proteins and pathogenesis mechanism of BBTV remains unclear. In this study, the coat protein gene (CP, ORF 516bp) and nuclear shuttle protein gene (NSP, ORF 465bp) from BBTV B2 isolate of the Southeast-Asia group were cloned. The intracellular localization analysis showed the CP locates in the cell nucleus of tobacco cells, while the NSP distributes in the cell nucleus and cytoplasm. Co-localization analysis indicated the NSP itself does not change distribution, but CP re-distributes to the cell nucleus and cytoplasm, suggesting that NSP interacts with CP and re-locates the CP in the cell. The interaction between CP and NSP was further verified by co-immunoprecipitation (Co-IP) in tobacco protoplasts. The study will help us to understand the interaction between viral proteins and pathogenesis mechanism of BBTV in host plants.

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